Rabbit Anti-Phospho-MEF2A (Thr312)/FITC Conjugated antibody
|别 名||MEF2A (phospho T312); p-MEF2A (phospho T312); MEF2A (Phospho Thr312); MEF2A (Phospho T312); MEF2A (Phospho-Thr312); myocyte enhancer-binding factor 2; ADCAD1; MADS box transcription enhancer factor 2 polypeptide A; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A); MEF 2A; MEF2; MEF2A; Myocyte enhancer factor 2A; RSRFC4; RSRFC9; Serum response factor like protein 1.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 免疫学 转录调节因子|
|交叉反应||Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||54kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human MEF2A around the phosphorylation site of Thr312|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.
MEF2A belongs to a family of DNA binding regulatory proteins. The MEF2 family of transcription factors is highly expressed in the brain when neurons undergo dendritic maturation and synapse formation. MEF2A is especially abundant in granule neurons of the cerebellar cortex throughout the period of synaptogenesis. MEF2A also has key roles in cardiac and skeletal muscle development.
Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
Binds DNA as a homo- or heterodimer. Dimerizes with MEF2D. Interacts with HDAC7 (By similarity). Interacts with PIAS1; the interaction enhances sumoylation. Interacts with HDAC4, HDAC9 and SLC2A4RG. Interacts (via the N-terminal) with MAPK7; the interaction results in the phosphorylation and transcriptional activity of MEF2A.
Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated with SUMO1 and, to a lesser extent with SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis (By similarity).
Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation
Defects in MEF2A are a cause of coronary artery disease, autosomal dominant, type 1 (ADCAD1)[MIM:608320]. A common heart disease characterized by reduced or absent blood flow in one or more of the arteries that encircle and supply the heart. Its most important complication is acute myocardial infarction.
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain.
Entrez Gene: 4205 Human
Entrez Gene: 17258 Mouse
Entrez Gene: 309957 Rat
Omim: 600660 Human
SwissProt: Q02078 Human
SwissProt: Q60929 Mouse
SwissProt: Q2MJT0 Rat
Unigene: 268675 Human
Unigene: 132788 Mouse
Unigene: 426509 Mouse
Unigene: 466976 Mouse
Unigene: 162435 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
从中胚层前体细胞向成肌细胞分化的过程导致发现了多种调节肌肉基因表达的组织特异性因子。肌源性碱性螺旋环螺旋蛋白，包括MyOD（MIM 159970）、肌生成蛋白（MIM 159980）、MYF5（MIM 159990）和MRF4（MIM 159991）是一类被鉴定的因子。第二家族的DNA结合调节蛋白是肌细胞特异性增强子因子-2（MEF2）家族。这些蛋白中的每一个与存在于许多（如果不是全部）肌肉特异性基因的调控区中的MEF2靶DNA序列结合。MEF2基因是MADS基因家族的成员（命名为酵母交配型特异转录因子MCM1）、植物同源基因“AgAm”和“缺陷”和人血清反应因子SRF（MIM 600589），该家族还包括几个同源异型基因和其他转录物。N因子，它们共享一个保守的DNA结合结构域。