FITC标记的磷酸化酶动力蛋白1抗体
产品名称： FITC标记的磷酸化酶动力蛋白1抗体
英文名称： Anti-phospho-Dynamin 1 (Ser774)/FITC
产品编号： HZ-13039R-FITC
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-phospho-Dynamin 1 (Ser774)/FITC Conjugated antibody
FITC标记的磷酸化酶动力蛋白1抗体
| 英文名称 | Anti-phospho-Dynamin 1 (Ser774)/FITC |
| 中文名称 | FITC标记的磷酸化酶动力蛋白1抗体 |
| 别 名 | Dynamin 1 (phospho S774); Dynamin 1 (phospho Ser774); p-Dynamin 1 (S774); p-Dynamin 1 (Ser774); B dynamin; D100; DNM 1; DNM; DNM1; DYN1_HUMAN; Dynamin; Dynamin-1; Dynamin1. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 神经生物学 信号转导 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 97kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Dynamin 1 around the phosphorylation site of Ser774 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: Dynamin I is a GTPase enzyme required for the retrieval of synaptic vesicles after exocytosis and functions in endocytosis by stimulating assembly of invaginating synaptic vesicles (1). Dynamin I is phosphorylated in nerve terminals exclusively in the cytosolic compartment and in vitro by protein kinase C (PKC) (2–5). The phosphorylation site in PKC-phosphorylated Dynamin I is a single site at Serine 795, which is located near a binding site for the SH3 domain of p85, the regulatory subunit of phosphatidylinositol 3-kinase (2–5). Dephosphorylation is required for synaptic vesicle retrieval, suggesting that phosphorylation affects the subcellular localization of Dynamin I (5). Mouse, rat and human Dynamin I are phosphorylated on serine residues, including Ser 778, by Cdk5, regulating PACSIN1 recruitment and enabling synaptic vesicle endocytosis. Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis. Subunit: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3 (By similarity). Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3 (By similarity). Interacts with SNX9. Interacts with MYO1E (via SH3 domain). Subcellular Location: Cytoplasm. Cytoplasm, cytoskeleton. Note=Microtubule-associated. Similarity: Belongs to the dynamin family. Contains 1 GED domain. Contains 1 PH domain. Database links: UniProtKB/Swiss-Prot: Q05193.2 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
Dynamin I是一种GTP酶，在胞吐后恢复突触小泡，并通过刺激内陷突触小泡的组装在胞吞过程中起作用(1)。Dynamin I在神经末梢中通过蛋白激酶C(PKC)(2-5)在胞浆室和体外被磷酸化。PKC-磷酸化Dynamin I的磷酸化位点是丝氨酸795的一个位点，位于p85的SH3结构域的结合位点附近，p85是磷脂酰肌醇3-激酶(2-5)的调节亚基。突触囊泡提取需要脱磷酸化，表明磷酸化影响Dynamin I(5)的亚细胞定位。小鼠、大鼠和人Dynamin I被Cdk5磷酸化在丝氨酸残基上，包括Ser 778，调节PACSIN1募集和使突触囊泡内吞。