FITC标记的磷酸化真核延伸因子激酶2抗体-抗体-抗体-生物在线
FITC标记的磷酸化真核延伸因子激酶2抗体

FITC标记的磷酸化真核延伸因子激酶2抗体

商家询价

产品名称: FITC标记的磷酸化真核延伸因子激酶2抗体

英文名称: Anti-Phospho-EEF2k (Ser359)/FITC

产品编号: HZ-13058R-FITC

产品价格: null

产品产地: 中国/上海

品牌商标: HZbscience

更新时间: 2023-08-17T10:24:20

使用范围: ICC=1:50-200 IF=1:50-200

上海沪震实业有限公司
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 Rabbit Anti-Phospho-EEF2k (Ser359)/FITC Conjugated antibody

FITC标记的磷酸化真核延伸因子激酶2抗体

 

英文名称 Anti-Phospho-EEF2k (Ser359)/FITC
中文名称 FITC标记的磷酸化真核延伸因子激酶2抗体
别    名 EEF2K (phospho S359); p-EEF2K (phospho S359); Calcium/calmodulin dependent eukaryotic elongation factor 2; Calcium/calmodulin dependent eukaryotic elongation factor 2 kinase; Calmodulin dependent protein kinase III; cb365; eEF 2 kinase; eEF 2K; EEF2K protein; Elongation factor 2 kinase; Eukaryotic elongation factor 2 kinase; kinase eEF2K; SMEF2K; EF2K_HUMAN.  
规格价格 100ul/2980元 购买        大包装/询价
说 明 书 100ul  
产品类型 磷酸化抗体 
研究领域 肿瘤  细胞生物  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Horse, Rabbit, Sheep, 
产品应用 ICC=1:50-200 IF=1:50-200  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 82kDa
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human EEF2k around the phosphorylation site of Ser359
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品介绍 background:
Eukaryotic elongation factor 2 kinase (EEF2k) previously known as Ca2+/calmodulin dependent protein kinase III, is an abundant cytoplasmic protein highly specific for elongation factor 2 (eEf2). Phosphorylation of eEF2 by eEF2 kinase on specific threonine residues results in the inactivation of eEF-2 and in termination of mRNA translation. The activity of eEF2 kinase is not only dependent upon Ca2+ ions, calmodulin (CaM) and insulin, but is also regulated both negatively and positively via phosphorylation by different protein kinases (AMPK, S6K1, p90 RSK). There is also evidence that eEF-2 phosphorylation is involved in the regulation of cell cycle progression, cellular differentiation, oogensis and malignant tumors.

Function:
Threonine kinase that regulates protein synthesis bycontrolling the rate of peptide chain elongation. Upon activationby a variety of upstream kinases including AMPK or TRM7,phosphorylates the elongation factor EEF2 at a single site, rendersit unable to bind ribosomes and thus inactive. In turn, the rate ofprotein synthesis is reduced.

Subunit:
Monomer or homodimer.

Post-translational modifications:
Autophosphorylated at multiple residues, Thr-348 being themajor site. Phosphorylated by AMP-activated protein kinase AMPK atSer-398 leading to EEF2K activation and protein synthesisinhibition. Phosphorylated by TRPM7 at Ser-78 resulting in improvedprotein stability, higher EE2F phosphorylated and subsequentlyreduced rate of protein synthesis. Phosphorylation by other kinasessuch as CDK1 and MAPK13 at Ser-359 or RPS6KA1 and RPS6KB1 atSer-366 instead decrease EEF2K activity and promote proteinsynthesis.

Similarity:
Belongs to the protein kinase superfamily.
Alpha-type protein kinase family.
Contains 1 alpha-type protein kinase domain. 

Database links:
UniProtKB/Swiss-Prot: O00418.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications

真核细胞伸长因子2激酶(EEF2k)以前称为Ca2+/钙调素依赖性蛋白激酶III,是一种丰富的细胞质蛋白,对伸长因子2(eEf2)具有高度特异性。eEF2激酶在特定苏氨酸残基上磷酸化eEF2导致eEF-2失活和mRNA翻译终止。eEF2激酶的活性不仅依赖于Ca2+离子、钙调素(CaM)和胰岛素,而且通过不同蛋白激酶(AMPK、S6K1、p90 RSK)的磷酸化作用负调节和正调节。也有证据表明,eEF-2磷酸化与细胞周期进程、细胞分化、卵子发生和恶性肿瘤的调节有关。