Rabbit Anti-phospho-Ephexin-1 (Tyr179)/FITC Conjugated antibody
|别 名||Ephexin-1 (phospho Y179); p-Ephexin-1 (phospho Y179); ARHGEF27; Eph-interacting exchange protein; EPHEXIN; Ephexin 1; Neuronal guanine nucleotide exchange factor; Ngef; NGEF_HUMAN.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 神经生物学 信号转导 G蛋白偶联受体 G蛋白信号|
|交叉反应||Human, Mouse, Rat, Chicken,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||82kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthesised phosphopeptide derived from human Ephexin-1 around the phosphorylation site of Tyr179|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
EPHEXIN is a 710 amino acid protein that localizes to both the membrane and the cytoplasm and contains one SH3 domain, one PH domain and one DH domain. Expressed at high levels in brain and present at lower levels in lung tissue, EPHEXIN interacts with EphA4 and functions as a guanine nucleotide exchange factor (GEF) that is capable of activating Rho A, Rac 1 and Cdc42 and is thought to play a role in axon guidance and growth cone collapse. EPHEXIN is subject to Src-dependent phosphorylation, an event that increases the GEF activity of EPHEXIN toward Rho A. Human EPHEXIN, which exists as multiple alternatively spliced isoforms, shares a high degree of sequence homology with its mouse counterpart, suggesting a conserved role between species.
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Interacts with CDK5R1 and EPHA4; activated by EPHA4 through the CDK5 kinase.
Cytoplasm. Membrane. Cell projection, growth cone. Associated with membranes. Localizes to axonal growth cones.
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
Database links : UniProtKB/Swiss-Prot: Q8N5V2.2
Entrez Gene: 25791 Human
Entrez Gene: 53972 Mouse
Entrez Gene: 246217 Rat
Entrez Gene: 424744 Chicken
Omim: 605991 Human
SwissProt: Q8N5V2 Human
SwissProt: Q8CHT1 Mouse
SwissProt: Q5BKC9 Rat
Unigene: 97316 Human
Unigene: 435439 Mouse
Unigene: 20670 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
EPHEXIN是一种710氨基酸蛋白，定位于细胞膜和细胞质，包含一个SH3结构域、一个PH结构域和一个DH结构域。EPHEXIN在脑中高水平表达，在肺组织中低水平表达，与EphA4相互作用，作为鸟嘌呤核苷酸交换因子（GEF）发挥作用，能够激活Rho A、Rac 1和Cdc42，被认为在轴突引导和生长锥体塌陷中起作用。EPHEXIN受Src依赖性***酸化的影响，这一事件增加了EPHEXIN对Rho A的GEF活性。作为多种选择性剪接异构体存在的人EPHEXIN与小鼠的EPHEXIN具有高度的序列同源性，表明sp之间的作用是保守的。爱西斯。