Rabbit Anti-KPNA1/FITC Conjugated antibody
|别 名||KPNA1; RCH2; SRP1; IMA5_HUMAN; Importin subunit alpha-5; Karyopherin subunit alpha-1; Nucleoprotein interactor 1; NPI-1; RAG cohort protein 2; SRP1-beta; Importin subunit alpha-5, N-terminally processed.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 染色质和核信号 信号转导|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||60kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human SRP1-beta|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. This protein interacts with the recombination activating gene 1 (RAG1) protein and is a putative substrate of the RAG1 ubiquitin ligase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012].
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
Heterodimer; with KPNB1. Interacts with ANP32E. Interacts with ZIC3 (By similarity). Interacts with the nucleoprotein of influenza A viruses. Binds to HCMV (human cytomegalovirus) UL84, HIV-1 Vpr and to ebolavirus VP24. Interacts with APEX1 and RAG1. Interacts with CTNNBL1 (via its N-terminal). Interacts with AICDA (via its NLS).
Polyubiquitinated in the presence of RAG1 (in vitro).
Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain.
Entrez Gene: 3836 Human
Entrez Gene: 16646 Mouse
Entrez Gene: 288064 Rat
Omim: 600686 Human
SwissProt: P52294 Human
SwissProt: Q60960 Mouse
SwissProt: P83953 Rat
Unigene: 161008 Human
Unigene: 6952 Mouse
Unigene: 6272 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
在真核细胞中，分子在细胞核和细胞质之间的运输是由核孔复合物（NPC）介导的，NPC由60-100种蛋白质组成。小分子（高达70kD）可以通过非选择性扩散穿过核孔，而大分子则通过活性过程运输。该基因编码的蛋白属于进口蛋白家族，参与细胞核蛋白质的导入。该蛋白与重组激活基因1（RAG1）蛋白相互作用，并且是RAG1泛素连接酶的推测底物。选择性剪接导致多个转录变体。[ RefSeq，NOV 2012 ]提供。