Anti-phospho-FHOD1 (Thr1141)抗体
产品名称： Anti-phospho-FHOD1 (Thr1141)抗体
英文名称： phospho-FHOD1 (Thr1141)
产品编号： YB--13159R
产品价格： null
产品产地： 中国/美国
品牌商标： Ybscience
更新时间： 2023-08-17T10:29:50
使用范围： 科研使用
上海钰博生物科技有限公司
- 联系人 : 陈环环
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- 邮编 : 200612
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Anti-phospho-FHOD1 (Thr1141)抗体
| 产品编号 | YB-13159R |
| 英文名称 | phospho-FHOD1 (Thr1141) |
| 中文名称 | 磷酸化肢体畸形相关蛋白FHOD1抗体 |
| 别 名 | FHOD1 (phospho T1141); p-FHOD1 (phospho T1141); FH1/FH2 domain containing protein; FH1/FH2 domain-containing protein 1; Fhod1; FHOD1_HUMAN; FHOS; FHOS1; Formin homolog overexpressed in spleen 1; Formin homology 2 domain containing 1; Formin homology 2 domain containing protein 1; Formin homology 2 domain-containing protein 1. |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 信号转导 结合蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 126kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human FHOD1 around the phosphorylation site of Thr1141:RR(p-T)LK |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The limb deformity (ld) locus influences normal limb development and gives rise to alternative mRNAs that can translate into a family of protein products known as formins. Formins play a crucial role in cytoskeletal reorganization by influencing actin filament assembly. The temporal genetic hierarchy influencing normal limb development can deregulate and mediate mammalian developmental syndromes. FHOD1 induces the formation of and associates with bundled actin stress fibers in response to the activity of the Rho-ROCK cascade. It influences several cellular activities including cell migration, cytoskeletal arrangement, signal transduction and gene expression. Function: Required for the assembly of F-actin structures, such as stress fibers. Depends on the Rho-ROCK cascade for its activity. Contributes to the coordination of microtubules with actin fibers and plays a role in cell elongation. Subcellular Location: Cytoplasm. Cytoplasm > cytoskeleton. Predominantly cytoplasmic. Tissue Specificity: Ubiquitous. Highly expressed in spleen. Similarity: Belongs to the formin homology family. Contains 1 FH1 (formin homology 1) domain. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. SWISS: Q9Y613 Gene ID: 29109 Database links: Entrez Gene: 29109 Human Omim: 606881 Human SwissProt: Q9Y613 Human Unigene: 95231 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |
 Sample:
293T(Human) Cell Lysate at 30 ug Primary: Anti- phospho-FHOD1 (Thr1141) (bs-13159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 126 kD Observed band size: 126 kD  Sample:
K562(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti- phospho-FHOD1 (Thr1141) (bs-13159R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 126 kD Observed band size: 126 kD  Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-phospho-FHOD1 Polyclonal Antibody, Unconjugated(bs-13159R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FHOD1 Polyclonal Antibody, Unconjugated(bs-13159R) 1:600, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |