产品名称： Anti-Frizzled 10/CD350抗体
英文名称： Frizzled 10/CD350
级 别： 分析纯, 见说明书
价 格： 1380元
抗原： KLH conjugated synthetic peptide derived from human Frizzled 10/CD350:101-200/581
适用物种： Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,
更新时间： 2018/10/11 11:40:00
|别 名||CD 350; CD 350 antigen; CD350; Frizzled (Drosophila) homolog 10; Frizzled-10; FZ 10; Fz-10; FZ10; FZD 10; FZD10; FZD10_HUMAN; FzE 7; FzE7; hFz 10; hFz10.|
|规格价格||100ul/1380元 购买 200ul/2200元 购买 大包装/询价|
|说 明 书||100ul 200ul|
|研究领域||肿瘤 细胞生物 信号转导 干细胞 G蛋白偶联受体 细胞表面分子 表观遗传学 G蛋白信号|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||68kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Frizzled 10/CD350:101-200/581 <Extracellular>|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
The frizzled gene, originally identified in Drosophila melanogaster, is involved in the development of tissue polarity. The mammalian homolog of frizzled as well as several secreted mammalian frizzled-related proteins (FRPs) have been described. The frizzled proteins contain seven transmembrane domains, a cysteine-rich domain in the extracellular region and a carboxy terminal Ser/Thr-xxx-Val motif. They function as receptors for Wnt and are generally coupled to G proteins. Upregulation of frizzled-10 mRNA in human cells may lead to carcinogenesis through Wnt- Beta-catenin-TCF signaling pathway activation. Frizzled-10 has been found to be upregulated in HeLa S3, NT2, TMK1 and MKN74 cancer cell lines as well as in colorectal and breast cancer.
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.
Highest levels in the placenta and fetal kidney, followed by fetal lung and brain. In adult brain, abundantly expressed in the cerebellum, followed by cerebral cortex, medulla and spinal cord; very low levels in total brain, frontal lobe, temporal lobe and putamen. Weak expression detected in adult brain, heart, lung, skeletal muscle, pancreas, spleen and prostate.
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.