Anti-TMEM176A抗体上海钰博生物科技有限公司

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产品名称: Anti-TMEM176A抗体
英文名称: TMEM176A
抗体货号: YB--13622R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from MOUSE TMEM176A:1-100/235
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 11:56:00
详细资料:  实验方法技术资料
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了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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上海钰博生物科技有限公司
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产品详细描述

 Anti-TMEM176A抗体

产品编号 YB-13622R
英文名称 TMEM176A
中文名称 跨膜蛋白176A抗体
别    名 GS188; HCA112; Hepatocellular carcinoma-associated antigen 112; likley ortholog of mouse GS188; T176A_MOUSE; Tmem176a; Transmembrane protein 176A.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  细胞生物  神经生物学  干细胞  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 26kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from MOUSE TMEM176A:1-100/235 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
TMEM176A is a 235 amino acid multi-pass membrane protein belonging to the TMEM176 family. The gene encoding GS188 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome. The deletion of a portion of the q arm of chromosome 7 is associated with Williams-Beuren syndrome, a condition characterized by mild mental retardation, an unusual comfort and friendliness with strangers and an elfin appearance. Deletions of portions of the q arm of chromosome 7 are also seen in a number of myeloid disorders including cases of acute myelogenous leukemia and myelodysplasia.

Subcellular Location:
Membrane.

Similarity:
Belongs to the TMEM176 family.

SWISS:
Q96HP8

Gene ID:
55365

Database links:

Entrez Gene: 55365 Human

Omim: 610334 Human

SwissProt: Q96HP8 Human

Unigene: 647116 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Protein: HepG2(human) lysates at 30ug; 
Primary: rabbit Anti-TMEM176A (bs-13622R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; 
Predicted band size:26 kD
Observed band size:26 kD
Protein: mouse kidney lysates, 30ug; 
Primary: Anti-TMEM176A (bs-13622R) at 1:300; 
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1:5000; 
ECL excitated the fluorescence; 
Predicted band size : 24kD
Observed band size : 24kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM176A) Polyclonal Antibody, Unconjugated (bs-13622R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-TMEM176A Polyclonal Antibody, Unconjugated(bs-13622R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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