Anti-CPNE1抗体上海钰博生物科技有限公司

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产品名称: Anti-CPNE1抗体
英文名称: CPNE1
抗体货号: YB--14024R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from human CPNE1:1-120/537
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, Pig, Cow, Sheep,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 12:51:00
详细资料:  实验方法技术资料
浏览人数:20
诚信指数:1476点
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使用范围:仅限科研使用,不能应用于临床
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上海钰博生物科技有限公司
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产品详细描述

 Anti-CPNE1抗体

产品编号 YB-14024R
英文名称 CPNE1
中文名称 钙依赖膜结合蛋白Copine蛋白1抗体
别    名 Copine 1; Copine I; Copine-1; Copine1; CopineI; COPN 1; COPN1; CPN 1; CPN1; CPNE 1; CPNE1; CPNE1_HUMAN; MGC1142.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  细胞生物  信号转导  转运蛋白  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Pig, Cow, Sheep, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 59kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CPNE1:1-120/537 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Copine I is a member of the copine family of evolutionarily conserved, soluble, calcium-dependent, membrane-binding proteins. Members of the copine family are involved in signal transduction and membrane trafficking. Copine I is ubiquitously expressed and contains two N-terminal C2 domains and one C-terminal VWFA (von Willebrand factor A) domain, which is also referred to as the A domain or the core domain. As is characteristic of the copine family, copine I functions in membrane trafficking and is capable of binding phospholipids in a calcium-dependent manner. Copine I is also able to bind liposomes. Via its VWFA domain, copine I directly interacts with the ubiquitin-conjugating enzyme, UBC12, and may play a role in the regulation of TNF alpha-signaling.

Function:
May function in membrane trafficking. Exhibits calcium-dependent phospholipid binding properties.

Subunit:
Interacts with CCDC22.

Similarity:
Belongs to the copine family.
Contains 2 C2 domains.
Contains 1 VWFA domain.

SWISS:
Q99829

Gene ID:
615677

Database links:

Entrez Gene: 615677 Cow

Entrez Gene: 477213 Dog

Entrez Gene: 8904 Human

Entrez Gene: 266692 Mouse

Entrez Gene: 100233174 Pig

Entrez Gene: 100328904 Rabbit

Omim: 604205 Human

SwissProt: Q99829 Human

SwissProt: Q8C166 Mouse

Unigene: 246413 Human

Unigene: 700188 Human

Unigene: 27660 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Protein: spleen(mouse) lysate at 40ug;
Primary: rabbit Anti-CPNE1 (bs-14024R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; 
Predicted band size: 59 kD
Observed band size: 59 kD
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-CPNE1 Polyclonal Antibody, Unconjugated(bs-14024R) 1:400, overnight at 4°C, followed by conjugation to the secondary 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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