Anti-NeuN抗体
产品名称: Anti-NeuN抗体
英文名称: NeuN
产品编号: YB--10394R
产品价格: null
产品产地: 中国/美国
品牌商标: Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: 科研使用
上海钰博生物科技有限公司
- 联系人 : 陈环环
- 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 183****2235
- 传真 : 021-60514606
- 邮箱 : shybio@126.com
Anti-NeuN抗体
产品编号 | YB-10394R |
英文名称 | NeuN |
中文名称 | 神经元核抗原抗体 |
别 名 | FOX3; NeuN; neuronal nuclei antigen; neuronal nuclei; Vertebrate neuron-specific nuclear protein; Neuna60; Neuronal nuclear antigen A60; RNA binding protein fox-1 homolog 3 isoform 1; RNA binding protein, fox 1 homolog (C. elegans) 3; Rbfox3; D11Bwg0517e; Fox-3; Fox3; Hrnbp3; RP23-159O6.5; novel protein; Fox-1 homolog C; fox1 homolog C; FLJ56884; FLJ58356; hexaribonucleotide binding protein 3 RFOX3_HUMAN. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 细胞生物 神经生物学 细胞凋亡 转运蛋白 细胞类型标志物 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Horse, Sheep, Bee, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 34kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human FOX3:101-200/312 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008] Function: RNA-binding protein that regulates alternative splicing events. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. Similarity: Contains 1 RRM (RNA recognition motif) domain. SWISS: A6NFN3 Gene ID: 146713 Database links: Entrez Gene: 146713 Human Entrez Gene: 52897 Mouse Entrez Gene: 287847 Rat SwissProt: A6NFN3 Human SwissProt: Q8BIF2 Mouse Unigene: 135229 Human Unigene: 341103 Mouse Unigene: 143966 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN/FOX3 Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NeuN Polyclonal Antibody, Unconjugated(bs-10394R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, AF488 conjugated(bs-0295G-AF488)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Blank control:A549.
Primary Antibody (green line): Rabbit Anti-NeuN antibody (bs-10394R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. |