Anti-LXR alpha抗体上海钰博生物科技有限公司

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产品名称: Anti-LXR alpha抗体
英文名称: LXR alpha
抗体货号: YB--10311R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from human LXR alpha:361-447/447
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, Chicken, Dog, Cow, Horse,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 12:57:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-LXR alpha抗体

产品编号 YB-10311R
英文名称 LXR alpha
中文名称 肝脏X受体抗体
别    名 Liver X receptor alpha; LXR a; LXRa; LXRA; NR1H3; NR1H3_HUMAN; Nuclear orphan receptor LXR alpha; Nuclear receptor subfamily 1 group H member 3; Oxysterols receptor LXR alpha; Oxysterols receptor LXR-alpha; RLD 1; RLD1.   
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 免疫学  细菌及病毒  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Cow, Horse, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 46kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human LXR alpha:361-447/447 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008].

Function:
Orphan receptor. Interaction with RXR shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8.

Subunit:
Heterodimer of LXRA and RXR.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Nucleus (Potential).

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily. 
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
Q13133

Gene ID:
10062

Database links:

Entrez Gene: 10062 Human

Entrez Gene: 22259 Mouse

Entrez Gene: 58852 Rat

Omim: 602423 Human

SwissProt: Q13133 Human

SwissProt: Q9Z0Y9 Mouse

SwissProt: Q62685 Rat

Unigene: 438863 Human

Unigene: 22690 Mouse

Unigene: 11209 Rat

 



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Protein: Huh7(human) lysates at 30ug;
Primary: Anti-LXR alpha (bs-10311R) at 1:500; 
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; 
ECL excitated the fluorescence; 
Predicted band size : 46 kD
Observed band size : 46 kD
Protein: liver(mouse) lysates at 40ug;
Primary: Anti-LXR alpha (bs-10311R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G-HRP) at 1: 5000; 
ECL excitated the fluorescence; 
Predicted band size : 46 kD
Observed band size : 46 kD
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-LXR alpha Polyclonal Antibody, Unconjugated(bs-10311R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Hela. 
Primary Antibody (green line): Rabbit Anti-LXR alpha antibody (bs-10311R) 
Dilution: 1μg /10^6 cells; 
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test. 
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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