Anti-Dopamine Receptor D1抗体上海钰博生物科技有限公司

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产品名称: Anti-Dopamine Receptor D1抗体
英文名称: Dopamine Receptor D1
抗体货号: YB-10610R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 780元
抗原: KLH conjugated synthetic peptide derived from human Dopamine Receptor D1:11-100/446
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Rat,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 13:08:00
详细资料:  实验方法技术资料
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诚信指数:1476点
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-Dopamine Receptor D1抗体

产品编号 YB-10610R
英文名称 Dopamine Receptor D1
中文名称 多巴胺受体D1抗体
别    名 DRD1; dopamine D1 receptor; D(1A) dopamine receptor; D1A dopamine receptor; Dopamine D1Receptors; D1DR; DADR; Dopamine Receptor D1; DR D1; DR D1A; DRD 1; DRD1 receptor; DRD1; DRD1A; DRD1_HUMAN; D(1A) dopamine receptor; DRD 1A; DRD1.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  免疫学  神经生物学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Dopamine Receptor D1:11-100/446 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Function:
Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.

Subunit:
Interacts with DNAJC14 via its C-terminus. Interacts with DRD1IP.

Subcellular Location:
Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Tissue Specificity:
Detected in caudate, nucleus accumbens and in the olfactory tubercle.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P21728

Gene ID:
1812

Database links:

Entrez Gene: 1812 Human

Entrez Gene: 13488 Mouse

Entrez Gene: 24316 Rat

Omim: 126449 Human

SwissProt: P21728 Human

SwissProt: Q61616 Mouse

SwissProt: P18901 Rat

Unigene: 2624 Human

Unigene: 54161 Mouse

Unigene: 24039 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample:A549 Cell(Human)Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 60kD
Sample:
A431 Cell(Human)Lysate at 40 ug
HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-Dopamine Receptor D1(bs-10610R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50kD
Observed band size: 60kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated(bs-10610R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Cell: SH-SY5Y
Concentration:1:100
Host/Isotype:Rabbit/IgG
Flow cytometric analysis of Rabbit IgG isotype control (Cat#: bs-10610R) on SH-SY5Y(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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