Anti-phospho-Smad2/Smad3 (Thr8)抗体-抗体-抗体-生物在线
Anti-phospho-Smad2/Smad3 (Thr8)抗体

Anti-phospho-Smad2/Smad3 (Thr8)抗体

商家询价

产品名称: Anti-phospho-Smad2/Smad3 (Thr8)抗体

英文名称: phospho-Smad2/Smad3 (Thr8)

产品编号: YB--8853R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

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 Anti-phospho-Smad2/Smad3 (Thr8)抗体

产品编号 YB-8853R
英文名称 phospho-Smad2/Smad3 (Thr8)
中文名称 磷酸化细胞信号转导分子SMAD2/SMAD3抗体
别    名 Smad2 + Smad3 (phospho T8); p-Smad2 + Smad3 (phospho T8); P-Smad2 /3 (phospho T8); hMAD 2; hMAD 3; hSMAD2; hSMAD3; Mad related protein 2; MADH2; MADH3; MADR2; Mothers against DPP homolog 2; Mothers against DPP homolog 3; Sma and Mad related protein 2; SMA and MAD related protein 3; SMAD 2; SMAD 3; SMAD family member 2; SMAD family member 3.  
规格价格 100ul/1580元 购买        大包装/询价
说 明 书 100ul  
产品类型 磷酸化抗体 
研究领域 肿瘤  细胞生物  免疫学  信号转导  细胞凋亡  转录调节因子  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 52kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthesised phosphopeptide derived from human Smad2/Smad3 around the phosphorylation site of Thr8:PF(p-T)PP 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]

Function:
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation.

Subcellular Location:
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.

SWISS:
P84022

Gene ID:
4087

Database links:

Entrez Gene: 4087 Human

Entrez Gene: 4088 Human

Entrez Gene: 17126 Mouse

Entrez Gene: 17127 Mouse

Entrez Gene: 25631 Rat

Entrez Gene: 29357 Rat

Omim: 601366 Human

Omim: 603109 Human

SwissProt: P84022 Human

SwissProt: Q15796 Human

SwissProt: Q62432 Mouse

SwissProt: Q8BUN5 Mouse

SwissProt: O70436 Rat

SwissProt: P84025 Rat

Unigene: 12253 Human

Unigene: 714621 Human

Unigene: 10636 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample:
cerebrum(mouse) Lysate at 40 ug
RSC96 cell(rat) Lysate at 30 ug
hepG2 cell(human) Lysate at 30 ug
Hcclm3 cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 48,52 kD
Sample:
U251 cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
Sample:
HUVEC cell(human) Lysate at 30 ug
Primary: Anti- p-Smad2/Smad3 (Thr8) (bs-8853R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52 kD
blank: A549 cells (blue line)
isotype control: rabbit IgG (orange line)
second antibody: goat anti-rabbit IgG (white blue line) 
primary antibody: rabbit Anti-phospho-Smad2/Smad3 (Thr8) (green line); contration: 3μg /10^6 cells