Anti-ADORA2B抗体
产品名称: Anti-ADORA2B抗体
英文名称: ADORA2B
产品编号: YB--10791R
产品价格: null
产品产地: 中国/美国
品牌商标: Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: 科研使用
上海钰博生物科技有限公司
- 联系人 : 陈环环
- 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 183****2235
- 传真 : 021-60514606
- 邮箱 : shybio@126.com
Anti-ADORA2B抗体
产品编号 | YB-10791R |
英文名称 | ADORA2B |
中文名称 | 腺苷A2b受体/神经生长因子1受体抗体 |
别 名 | Adenosine A2b Receptor; adora 2; adora 2b; ADORA2; ADORA2B; HGNC:264; Netrin 1 receptor; AA2BR_HUMAN. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 肿瘤 心血管 免疫学 染色质和核信号 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Dog, Cow, Rabbit, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 37kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human ADORA2B:21-120/332 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008]. Function: Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P29275 Gene ID: 136 Database links: Entrez Gene: 136 Human Omim: 600446 Human SwissProt: P29275 Human Unigene: 167046 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Protein: HT29(human) lysate at 40ug;
Primary: rabbit Anti-ADORA2B (bs-10791R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 37 kD Observed band size: 37 kD Sample: HT29 (human)cell Lysate at 40 ug
Primary: Anti-ADORA2B(bs-10791R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37kD Observed band size: 37kD Protein: brain(mouse) lysate at 40ug;
Primary: rabbit Anti-ADORA2B (bs-10791R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; Predicted band size: 37 kD Observed band size: 37 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10791R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |