Anti-ADORA2B抗体上海钰博生物科技有限公司

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产品名称: Anti-ADORA2B抗体
英文名称: ADORA2B
抗体货号: YB--10791R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from human ADORA2B:21-120/332
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, Dog, Cow, Rabbit,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/12/15 13:50:00
详细资料:  实验方法技术资料
浏览人数:21
诚信指数:1476点
了解更多:进入公司展台
使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-ADORA2B抗体

产品编号 YB-10791R
英文名称 ADORA2B
中文名称 腺苷A2b受体/神经生长因子1受体抗体
别    名 Adenosine A2b Receptor; adora 2; adora 2b; ADORA2; ADORA2B; HGNC:264; Netrin 1 receptor; AA2BR_HUMAN.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  心血管  免疫学  染色质和核信号  信号转导  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 37kDa
细胞定位 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ADORA2B:21-120/332 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes an adenosine receptor that is a member of the G protein-coupled receptor superfamily. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation. The gene is located near the Smith-Magenis syndrome region on chromosome 17. [provided by RefSeq, Jul 2008].

Function:
Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subcellular Location:
Cell membrane; Multi-pass membrane protein.

Similarity:
Belongs to the G-protein coupled receptor 1 family.

SWISS:
P29275

Gene ID:
136

Database links:

Entrez Gene: 136 Human

Omim: 600446 Human

SwissProt: P29275 Human

Unigene: 167046 Human



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Protein: HT29(human) lysate at 40ug; 
Primary: rabbit Anti-ADORA2B (bs-10791R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; 
Predicted band size: 37 kD
Observed band size: 37 kD
Sample: HT29 (human)cell Lysate at 40 ug
Primary: Anti-ADORA2B(bs-10791R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 37kD
Observed band size: 37kD
Protein: brain(mouse) lysate at 40ug; 
Primary: rabbit Anti-ADORA2B (bs-10791R) at 1:300; 
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000; 
Predicted band size: 37 kD
Observed band size: 37 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-ADORA2B Polyclonal Antibody, Unconjugated(bs-10791R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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