级 别： 分析纯, 见说明书
价 格： 1380元
抗原： KLH conjugated synthetic peptide derived from human Anillin:621-720/1124
适用物种： Human, Mouse, Rat, Dog, Horse,
更新时间： 2019/4/19 15:08:00
|别 名||Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Anillin actin binding protein; ANLN; DKFZp779A055; Scra; Scraps; Scraps homolog.|
|规格价格||100ul/1380元 购买 200ul/2200元 购买 大包装/询价|
|说 明 书||100ul 200ul|
|研究领域||细胞生物 细胞周期蛋白 结合蛋白 细胞骨架|
|交叉反应||Human, Mouse, Rat, Dog, Horse,|
|产品应用||WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||124kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Anillin:621-720/1124|
|纯化方法||affinity purified by Protein A|
|储 存 液||Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.
Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.
Contains 1 PH domain.
Entrez Gene: 54443 Human
Entrez Gene: 68743 Mouse
Entrez Gene: 363031 Rat
SwissProt: Q9NQW6 Human
SwissProt: Q8K298 Mouse
Unigene: 62180 Human
Unigene: 282751 Mouse
Unigene: 453238 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.