Anti-c-fos抗体-抗体-抗体-生物在线
Anti-c-fos抗体

Anti-c-fos抗体

商家询价

产品名称: Anti-c-fos抗体

英文名称: c-fos

产品编号: YB--23041R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

上海钰博生物科技有限公司
  • 联系人 : 陈环环
  • 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 183****2235
  • 传真 : 021-60514606
  • 邮箱 : shybio@126.com

 Anti-c-fos抗体

产品编号 YB-23041R
英文名称 c-fos
中文名称 c-fos抗体
别    名 Cellular oncogene fos; FBJ murine osteosarcoma viral v fos oncogene homolog antibody FBJ Osteosarcoma Virus; FOS; FOS protein; G0 G1 switch regulatory protein 7; G0S7; Oncogene FOS; Proto oncogene protein c fos; v fos FBJ murine osteosarcoma viral oncogene homolog; AP-1; p55; FOS_HUMAN; Proto-oncogene c-Fos; G0/G1 switch regulatory protein 7.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  细胞生物  免疫学  神经生物学  信号转导  转录调节因子  肿瘤细胞生物标志物  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, 
产品应用 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 41kDa
细胞定位 细胞核 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human c-fos:331-380/380 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008].

Function:
Nuclear phosphoprotein which forms a tight butnon-covalently linked complex with the JUN/AP-1 transcriptionfactor. In the heterodimer, FOS and JUN/AP-1 basic regions eachseems to interact with symmetrical DNA half sites. On TGF-betaactivation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at theAP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Hasa critical function in regulating the Has a critical function inregulating the development of cells destined to form and maintainthe skeleton. It is thought to have an important role in signaltransduction, cell proliferation and differentiation.

Subunit:
Heterodimer; with JUN (By similarity). Interacts withMAFB. Component of the SMAD3/SMAD4/JUN/FOS complexrequired for syngernistic TGF-beta-mediated transcription at theAP1 promoter site. Interacts with SMAD3; the interaction is weakeven on TGF-beta activation. Interacts with MAFB. Interacts withDSIPI; this interaction inhibits the binding of active AP1 to itstarget DNA.

Subcellular Location:
Nucleus.

Post-translational modifications:
Phosphorylated in the C-terminal upon stimulation by nervegrowth factor (NGF) and epidermal growth factor (EGF).Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on bothSer-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to proteinstabilization with phosphorylation on Ser-374 being the major sitefor protein stabilization on NGF stimulation. Phosphorylation onSer-362 and Ser-374 primes further phosphorylations on Thr-325 andThr-331 through promoting docking of MAPK to the DEF domain.Phosphorylation on Thr-232, induced by HA-RAS, activates thetranscriptional activity and antagonizes sumoylation.Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes toosteoblast transformation (By similarity). [PTM] Constitutively sumoylated by SUMO1, SUMO2 and SUMO3.Desumoylated by SENP2. Sumoylation requires heterodimerization withJUN and is enhanced by mitogen stimulation. Sumoylation inhibitsthe AP-1 transcriptional activity and is, itself, inhibited byRas-activated phosphorylation on Thr-232.

Similarity:
Belongs to the bZIP family. Fos subfamily.
Contains 1 bZIP domain.

SWISS:
P01100

Gene ID:
2353

Database links:

Entrez Gene: 2353 Human

Entrez Gene: 14281 Mouse

Entrez Gene: 314322 Rat

Omim: 164810 Human

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Unigene: 246513 Mouse

Unigene: 103750 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

c-fos的作用主要用于各种类型的恶性肿瘤如食管癌、鼻咽癌、乳腺癌、结肠癌以及脑病的研究。 c-fos原癌基因及其蛋白产物不仅参与细胞的正常生长、分化过程,而且也参与细胞内信息传递过程和细胞的能量代谢过程,对细胞的增生、分化、转化都有调节作用、在生命活动中起着极为基础而重要的作用。
产品图片
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-23041R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-23041R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (c-fos) Polyclonal Antibody, Unconjugated (bs-23041R) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Blank control (blue line): Hela cells (fixed with 70% methanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃).
Primary Antibody (green line): Rabbit Anti-c-fos antibody (bs-23041R),Dilution: 1μg /10^6 cells; 
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE<,Dilution: 1μg /test.