Anti-GPR49/LGR5抗体上海钰博生物科技有限公司

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产品名称: Anti-GPR49/LGR5抗体
英文名称: 英文名称
抗体货号: YB--20747R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from human GPR49/LGR5:211-310/907
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 14:05:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-GPR49/LGR5抗体

产品编号 YB-20747R
英文名称 GPR49/LGR5
中文名称 G蛋白偶联受体49抗体
别    名 G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR49; GPR67; Leucine rich repeat containing G protein coupled receptor 5; LGR5; HG 38; HG38; LGR5; LGR5_HUMAN; MGC117008; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; Orphan G protein coupled receptor HG38.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  信号转导  干细胞  细胞凋亡  G蛋白偶联受体  G蛋白信号  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 98kDa
细胞定位 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human GPR49/LGR5:211-310/907 <Extracellular>
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Function:
Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development.

Subunit:
Identified in a complex composed of RNF43, LGR5 and RSPO1.

Subcellular Location:
Cell membrane; Multi-pass membrane protein

Tissue Specificity:
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.

Similarity:
Belongs to the G-protein coupled receptor 1 family.
Contains 16 LRR (leucine-rich) repeats.
Contains 1 LRRNT domain.

SWISS:
O75473

Gene ID:
8549

Database links:

Entrez Gene: 8549 Human

Entrez Gene: 14160 Mouse

Entrez Gene: 299802 Rat

Omim: 606667 Human

SwissProt: O75473 Human

SwissProt: Q9Z1P4 Mouse

Unigene: 658889 Human

Unigene: 42103 Mouse

Unigene: 214063 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

Lgr5基因(Wnt细胞信号系统)是一种G蛋白偶联受体,已经被确定为几种成年组织和癌症中干细胞的一个独特标记。Lgr5最初是在结肠癌细胞中发现的,有报道称:在恶化前的小鼠腺瘤中也有发现,这说明Lgr5很可能也是其他组织成体干细胞和癌症干细胞的标志物。
产品图片
Sample: 
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-LGR5 (bs-20747R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (bs-20747R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (bs-20747R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control (blue line): U251(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃)
Primary Antibody (green line): Rabbit Anti-GPR49 antibody (bs-20747R),Dilution: 1μg /10^6 cells; 
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. 

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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