Anti-GFAP抗体上海钰博生物科技有限公司

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产品名称: Anti-GFAP抗体
英文名称: GFAP
抗体货号: YB--33065M
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 860元
抗原: Recombinant mouse GFAP full length:
抗原来源: Mouse
抗体来源: Mouse
适用物种: Mouse, Rat,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 14:16:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-GFAP抗体

产品编号 YB-33065M
英文名称 GFAP
中文名称 胶质纤维酸性蛋白单克隆抗体
别    名 Astrocyte; FLJ45472; GFAP; Glial Fibrillary Acidic Protein; Intermediate filament protein; GFAP_HUMAN.  
规格价格 50ul/860元 购买    100ul/1580元 购买        大包装/询价
说 明 书 50ul  100ul  
研究领域 肿瘤  细胞生物  神经生物学  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 7D8
交叉反应 Mouse, Rat, 
产品应用 IHC-P=1:400-800 IHC-F=1:400-800 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 49kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 Recombinant mouse GFAP full length: 
亚    型 IgG
纯化方法 affinity purified by Protein G
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Function:
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.

Subunit:
Interacts with SYNM. Isoform 3 interacts with PSEN1 (via N-terminus).

Subcellular Location:
Cytoplasm. Note=Associated with intermediate filaments.

Tissue Specificity:
Expressed in cells lacking fibronectin.

Post-translational modifications:
Phosphorylated by PKN1.

DISEASE:
Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.

Similarity:
Belongs to the intermediate filament family.

SWISS:
P14136

Gene ID:
2670

Database links:

Entrez Gene: 281189 Cow

Entrez Gene: 2670 Human

Entrez Gene: 14580 Mouse

Entrez Gene: 24387 Rat

Omim: 137780 Human

SwissProt: Q28115 Cow

SwissProt: P14136 Human

SwissProt: P03995 Mouse



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

星形胶质细胞标志物 (Astrocyte Marker) 
GFAP是一个56kDa的中间丝蛋白(intermediate filament,IF),在中枢神经系统发育期是一个特异性的标志物,以区别星形细胞和其它胶质细胞。GFAP表达在皮层和海马,急、慢性皮质***治疗时表达减少。
GFAP可以和人、大鼠、小鼠的GFAP反应,在正常和肿瘤性的星形胶质细胞阳性表达,而神经节细胞、神经元、成纤维细胞、少突胶质细胞和这些细胞来源的肿瘤细胞阴性表达,主要用于星形胶质瘤等中枢神经系统肿瘤的诊断和鉴别诊断,GFAP的缺乏可导致AD病。
产品图片
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GFAP) Monoclonal Antibody, Unconjugated (bsm-33065M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

生物在线声明:以上所展示的信息由企业自行提供,内容的真实性、准确性和合法性由发布企业负责。生物在线对此不承担任何保证责任。

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