Anti-ALOX5/5 lipoxygenase抗体上海钰博生物科技有限公司

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产品名称: Anti-ALOX5/5 lipoxygenase抗体
英文名称: ALOX5/5 lipoxygenase
抗体货号: YB--0526R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 780元
抗原: KLH conjugated synthetic peptide derived from human 5 Lipoxygenase:601-674/674
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/10/11 14:33:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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产品详细描述

 Anti-ALOX5/5 lipoxygenase抗体

产品编号 YB-0526R
英文名称 ALOX5/5 lipoxygenase
中文名称 5-脂氧合酶抗体
别    名 5-lipoxygenase; 5-LOX; 5 LO; 5LPG; ALOX 5; ALOX5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN.  
Specific References  (2)     |     bs-0526R has been referenced in 2 publications.
[IF=0.00] Wang, Ruiqi, et al. "The comparative study of acetyl-11-keto-beta-boswellic acid (AKBA) and aspirin in the prevention of intestinal adenomatous polyposis in APC Min/+ mice." Drug Discoveries & Therapeutics 8.1 (2014).  IHC-P ;  Mouse.  
PubMed:24647155
[IF=1.72] Liang, Guojuan, et al. "The protective effect of caffeic acid on global cerebral ischemia-reperfusion injury in rats." Behavioral and Brain Functions 11.1 (2015): 1.  WB ;  Rat.  
PubMed:25907417
 
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  免疫学  转录调节因子  激酶和***酸酶  合成与降解  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 78kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human 5 Lipoxygenase:601-674/674 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined.

Function:
Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Subunit:
Interacts with ALOX5AP and LTC4S.

Subcellular Location:
Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. 

Post-translational modifications:
Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus.

Similarity:
Belongs to the lipoxygenase family.
Contains 1 lipoxygenase domain.
Contains 1 PLAT domain.

SWISS:
P48999

Gene ID:
240

Database links:

Entrez Gene: 477753 Dog

Entrez Gene: 240 Human

Entrez Gene: 11689 Mouse

Entrez Gene: 25290 Rat

Omim: 152390 Human

SwissProt: P09917 Human

SwissProt: P48999 Mouse

SwissProt: P12527 Rat

Unigene: 89499 Human

Unigene: 41072 Mouse

Unigene: 9662 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

合成与降解(Synthesis and Degradation)
5-脂氧合酶蛋白是花生四烯酸代谢途径中的一种关键酶,在恶性肿瘤的发生、发展过程中起了重要作用。抑制5-脂氧合酶及其产物的表达有可能预防和逆转恶性肿瘤的发生。
产品图片
Sample: 
Cerebrum (Rat) Lysate at 40 ug
Intestine (Mouse) Lysate at 40 ug
Primary: Anti-5 lipoxygenase (bs-0526R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 78 kD
Observed band size: 40,78 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALOX5 5 lipoxygenase) Polyclonal Antibody, Unconjugated (bs-0526R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-5 lipoxygenase Polyclonal Antibody, Unconjugated(bs-0526R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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