FITC标记的白喉毒素B抗体
产品名称: FITC标记的白喉毒素B抗体
英文名称: Anti-Diphtheria toxin fragment B/FITC
产品编号: HZ-10668R-FITC
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: ICC=1:50-200 IF=1:50-200
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Rabbit Anti-Diphtheria toxin fragment B/FITC Conjugated antibody
FITC标记的白喉毒素B抗体
英文名称 | Anti-Diphtheria toxin fragment B/FITC |
中文名称 | FITC标记的白喉毒素B抗体 |
别 名 | Diphtheria Toxin; DT; NAD(+)--diphthamide ADP-ribosyltransferase; Diphtheria toxin fragment B; DTX_CORBE. |
规格价格 | 100ul/2980元 购买 大包装/询价 |
说 明 书 | 100ul |
研究领域 | |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Corynephage beta |
产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 38kDa |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human Diphtheria toxin fragment B |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
产品介绍 | background: Diphtheria toxin is a 58 kDa protein secreted by lysogenic strains of Corynebacterium diphtheriae. The toxin causes the disease diphtheria in humans by gaining entry into the cell cytoplasm and inhibiting protein synthesis. The mechanism of inhibition involves transfer of the ADP-ribose group of NAD to elongation factor-2 (EF-2), rendering EF-2 inactive. The catalysed reaction is as follows: NAD + + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide The crystal structure of the diphtheria toxin homodimer has been determined to 2.5A resolution. The structure reveals a Y-shaped molecule of 3 domains, a catalytic domain (fragment A), whose fold is of the alpha + beta type; a transmembrane (TM) domain, which consists of 9 alpha-helices, 2 pairs of which may participate in pH-triggered membrane insertion and translocation; and a receptor-binding domain, which forms a flattened beta-barrel with a jelly-roll-like topology. The TM- and receptor binding-domains together constitute fragment B. Function: Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to eukaryotic elongation factor 2 (eEF-2). Fragment A is the catalytic portion responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A. Subcellular Location: Homodimer. Database links: Entrez Gene: 2650491 CORBE Swiss Prot: P00588 CORBE Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
白喉毒素是由白喉棒状杆菌裂解菌株分泌的58 kDa蛋白。毒素通过进入细胞胞浆和抑制蛋白质合成而导致人类白喉症。其抑制机制涉及NAD的ADP核糖基转移到伸长因子-2(EF-2),使EF-2失活。催化反应为:NAD++肽二苯二甲酰胺=烟酰胺+肽N-(ADP-D-核糖基)二苯二甲酰胺。该结构显示了一个Y形分子,包括3个结构域,一个催化结构域(片段A),其折叠为α+β型;一个跨膜(TM)结构域,它由9个α螺旋组成,其中2对可能参与pH触发的膜插入和易位;以及一个受体结合。IN域,它形成一个扁平的β桶,具有果冻卷状拓扑结构。Tm和受体结合域共同构成B片段。