Anti-XRCC1抗体-抗体-抗体-生物在线
Anti-XRCC1抗体

Anti-XRCC1抗体

商家询价

产品名称: Anti-XRCC1抗体

英文名称: XRCC1

产品编号: YB--1218R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

上海钰博生物科技有限公司
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 Anti-XRCC1抗体

产品编号 YB-1218R
英文名称 XRCC1
中文名称 X射线修复交叉互补蛋白抗体
别    名 DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray repair complementing defective repair in chinese hamster cells 1; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair cross complementing protein 1; RCC; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN; XRCC 1.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 肿瘤  染色质和核信号  新陈代谢  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 69kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human XRCC1:1-120/633 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
XRCC1 is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. In testis XRCC1 is expressed at high levels.

Function:
Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.

Subunit:
Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1. 

Subcellular Location:
Nucleus. Note=Accumulates at sites of DNA damage.

Post-translational modifications:
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.
Sumoylated.

Similarity:
Contains 2 BRCT domains.

SWISS:
P18887

Gene ID:
7515

Database links:

Entrez Gene: 7515 Human

Entrez Gene: 22594 Mouse

Entrez Gene: 84495 Rat

Omim: 194360 Human

SwissProt: P18887 Human

SwissProt: Q60596 Mouse

SwissProt: Q9ESZ0 Rat

Unigene: 98493 Human

Unigene: 4347 Mouse

Unigene: 13754 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

XRCC-1是一种重要的DNA修复基因,在一系列内外氧化剂(包括烟草成分)导致的DNA单链断裂和碱基损伤修复中起到重要作用,是目前肿瘤研究的重要的蛋白。
产品图片
Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-XRCC1 Polyclonal Antibody, Unconjugated(bs-1218R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining