Anti-phospho-KLF5 (Ser272)抗体-抗体-抗体-生物在线
Anti-phospho-KLF5 (Ser272)抗体

Anti-phospho-KLF5 (Ser272)抗体

商家询价

产品名称: Anti-phospho-KLF5 (Ser272)抗体

英文名称: phospho-KLF5 (Ser272)

产品编号: YB--2019R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

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 Anti-phospho-KLF5 (Ser272)抗体

产品编号 YB-2019R
英文名称 phospho-KLF5 (Ser272)
中文名称 磷酸化KLF5抗体
别    名 Klf5; CKLF; Krueppel-like factor 5; Intestinal-enriched krueppel-like factor; Transcription factor BTEB2; Basic transcription element-binding protein 2; BTE-binding protein 2; Krueppel-like factor 5; KLF5; basic transcription element-binding protein 2;BTEB2; intestine enriched krüppel-like factor; IKLF; Kruppel like factor 5; Transcription factor BTEB 2; Transcription factor BTEB2; KLF5_HUMAN .  
规格价格 100ul/1580元 购买        大包装/询价
说 明 书 100ul  
产品类型 磷酸化抗体 
研究领域 细胞生物  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 50kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Klf5 around the phosphorylation site of Ser272:PP(p-S)PP 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Krüppel-like factors (KLFs) zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation. KLF5 protein is enriched in intestinal tissue.

Function:
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Subunit:
Interacts with WWP1.

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed only in testis and placenta.

Post-translational modifications:
Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.

Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family. 
Contains 3 C2H2-type zinc fingers.

SWISS:
Q13887

Gene ID:
688

Database links:

Entrez Gene: 535702 Cow

Entrez Gene: 688 Human

Entrez Gene: 12224 Mouse

Entrez Gene: 100038005 Pig

Entrez Gene: 84410 Rat

Omim: 602903 Human

SwissProt: Q13887 Human

SwissProt: Q9Z0Z7 Mouse

Unigene: 508234 Human

Unigene: 30262 Mouse

Unigene: 8954 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-phospho-KLF5(Ser272) Polyclonal Antibody, Unconjugated(bs-2019R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining