Anti-CK20抗体-抗体-抗体-生物在线
Anti-CK20抗体

Anti-CK20抗体

商家询价

产品名称: Anti-CK20抗体

英文名称: CK20

产品编号: YB--1588R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

上海钰博生物科技有限公司
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 Anti-CK20抗体

产品编号 YB-1588R
英文名称 CK20
中文名称 细胞角蛋白20抗体
别    名 Cytokeratin 20; CK 20; CK20; Cytokeratin20; K20; Keratin 20; Keratin type I cytoskeletal 20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; Protein IT; K1C20_HUMAN.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 肿瘤  免疫学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 48kDa
细胞定位 细胞浆 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CK20:151-250/424 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.

Function:
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).

Subunit:
Heterotetramer of two type I and two type II keratins. Associates with KRT8.

Subcellular Location:
Cytoplasm.

Tissue Specificity:
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.

Post-translational modifications:
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). 
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. 

Similarity:
Belongs to the intermediate filament family.

SWISS:
P35900

Gene ID:
54474

Database links:

Entrez Gene: 54474 Human

Entrez Gene: 66809 Mouse

Entrez Gene: 286912 Rat

Omim: 608218 Human

SwissProt: P35900 Human

SwissProt: Q9D312 Mouse

SwissProt: P25030 Rat

Unigene: 84905 Human

Unigene: 28042 Mouse

Unigene: 9887 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

结构蛋白(Structural Proteins)
CK-20细胞角蛋白-20主要用于肿瘤方面的研究,近年来大多用于消化系统恶性肿瘤的鉴别,主要在胞浆。
产品图片
Sample: 
Large intestine (Mouse) Lysate at 40 ug
Primary: Anti-CK20 (bs-1588R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD