Anti-SV2A抗体-抗体-抗体-生物在线
Anti-SV2A抗体

Anti-SV2A抗体

商家询价

产品名称: Anti-SV2A抗体

英文名称: SV2A

产品编号: YB--2407R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

上海钰博生物科技有限公司
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 Anti-SV2A抗体

产品编号 YB-2407R
英文名称 SV2A
中文名称 突触泡蛋白2A抗体
别    名 Synaptic vesicle protein 2A; Synaptic vesicle glycoprotein 2A; Synaptic vesicle protein 2A; Synaptic vesicle protein 2; Sv2; Kiaa0736; SV2A_HUMAN.  
规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 50ul  100ul  200ul
研究领域 神经生物学  细胞膜受体  糖蛋白  线粒体  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 ELISA=1:500-1000 Flow-Cyt=1ug/test  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 83kDa
细胞定位 细胞浆 细胞膜 线粒体
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human SV2A:451-550/743 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
SV2s (Synaptic Vesicle protein 2) are integral membrane glycoproteins present in all synaptic vesicles. They have 12 transmembrane domains predicted by sequence analysis. There are three characterized isoforms, SV2A, SV2B and SV2C. SV2A is expressed ubiquitously throughout the brain. SV2B has a more restricted distribution with varying degrees of coexpression with SV2A. SV2C is more closely related to SV2A but shows a very restricted expression pattern. The highest expression levels were observed in phylogenetically old brain areas like pallidum, the midbrain and the olfactory bulb. SV2A is probably involved in the maintenance of a pool of synaptic vesicles competent for calcium-stimulated exocytosis. SV2A is a highly glycosylated synaptic vesicle protein with homology to transmembrane transporters.

Function:
Plays a role in the control of regulated secretion in neural and endocrine cells, enhancing selectively low-frequency neurotransmission. Positively regulates vesicle fusion by maintaining the readily releasable pool of secretory vesicles (By similarity).

Subunit:
Interacts with SYT1/synaptotagmin-1 in a calcium-dependent manner. Binds the adapter protein complex AP-2.

Subcellular Location:
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein.

Post-translational modifications:
Phosphorylation by CK1 of the N-terminal cytoplasmic domain regulates interaction with SYT1.
N-glycosylated.

Similarity:
Belongs to the major facilitator superfamily.

SWISS:
Q7L0J3

Gene ID:
9900

Database links:

Entrez Gene: 9900 Human

Entrez Gene: 64051 Mouse

Entrez Gene: 117559 Rat

Omim: 185860 Human

SwissProt: Q29397 Cow

SwissProt: Q7L0J3 Human

SwissProt: Q9JIS5 Mouse

SwissProt: Q02563 Rat

Unigene: 516153 Human

Unigene: 200365 Mouse

Unigene: 11264 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

突触泡蛋白2A(SV2A)是一类跨膜糖蛋白,定位于脊椎动物神经元及内分泌细胞,与神经递质的释放、内分泌泡胞吐作用、突触泡稳态的维持作用。
产品图片
Blank control:Molt-4. 
Primary Antibody (green line): Rabbit Anti-SV2A antibody (bs-2407) 
Dilution: 1μg /10^6 cells; 
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test. 
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.