Anti-HIRA抗体-抗体-抗体-生物在线
Anti-HIRA抗体

Anti-HIRA抗体

商家询价

产品名称: Anti-HIRA抗体

英文名称: HIRA

产品编号: YB--3530R

产品价格: null

产品产地: 中国/美国

品牌商标: Ybscience

更新时间: 2023-08-17T10:29:50

使用范围: 科研使用

上海钰博生物科技有限公司
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  • 所在区域 : 上海
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  • 邮箱 : shybio@126.com

Anti-HIRA抗体

产品编号 YB-3530R
英文名称 HIRA
中文名称 组蛋白替换精蛋白DGGR1抗体
别    名 DGCR1; DGGR 1; DGGR1; DiGeorge critical region gene 1; HIR; HIR histone cell cycle regulation defective homolog A; HIRA; HIRA protein; HIRA_HUMAN; Protein HIRA; TUP 1; TUP1; TUP1 like enhancer of split protein 1; TUP1-like enhancer of split protein 1; TUPLE 1; TUPLE1.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 细胞生物  免疫学  发育生物学  染色质和核信号  干细胞  通道蛋白  表观遗传学  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, 
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 112kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human HIRA/DGGR1:251-350/1017 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
This gene encodes a histone chaperone that preferentially places the variant histone H3.3 in nucleosomes. Orthologs of this gene in yeast, flies, and plants are necessary for the formation of transcriptionally silent heterochomatin. This gene plays an important role in the formation of the senescence-associated heterochromatin foci. These foci likely mediate the irreversible cell cycle changes that occur in senescent cells. It is considered the primary candidate gene in some haploinsufficiency syndromes such as DiGeorge syndrome, and insufficient production of the gene may disrupt normal embryonic development. [provided by RefSeq, Jul 2008]

Function:
Cooperates with ASF1A to promote replication-independent chromatin assembly. Required for the periodic repression of histone gene transcription during the cell cycle. Required for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

Subunit:
Interacts with histone H3F3B, PAX3 and PAX7 (By similarity). Interacts with CCNA1, HIRIP3, NFU1/HIRIP5 and histone H2B. Part of a complex which includes ASF1A, CABIN1, histone H3.3, histone H4 and UBN1.

Subcellular Location:
Nucleus. Nucleus, PML body. Note=Primarily, though not exclusively, localized to the nucleus. Localizes to PML bodies immediately prior to onset of senescence. 

Tissue Specificity:
Expressed at high levels in kidney, pancreas and skeletal muscle and at lower levels in brain, heart, liver, lung, and placenta.

Post-translational modifications:
Sumoylated.
Phosphorylated by CDK2/CCNA1 and CDK2/CCNE1 on Thr-555 in vitro. Also phosphorylated on Thr-555 and Ser-687 in vivo.

Similarity:
Belongs to the WD repeat HIR1 family. 
Contains 8 WD repeats.

SWISS:
P54198

Gene ID:
7290

Database links:

Entrez Gene: 7290 Human

Entrez Gene: 15260 Mouse

Entrez Gene: 363849 Rat

Omim: 600237 Human

SwissProt: P54198 Human

SwissProt: Q61666 Mouse

Unigene: 474206 Human

Unigene: 15694 Mouse

Unigene: 453041 Mouse

Unigene: 14920 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Sample: Pancreas (Mouse) Lysate at 40 ug
Primary: Anti-HIRA (bs-3530R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 112 kD
Observed band size: 112 kD