Rabbit Anti-Tubulin alpha/FITC Conjugated antibody
|别 名||TUBA1A + TUBA1B; Tubulin, Alpha 1b; Tubulin Alpha-Ubiquitous Chain; Alpha-Tubulin Ubiquitous; Tubulin K-Alpha-1; Tubulin, Alpha, Ubiquitous; Tubulin Alpha-1B Chain; Alpha Tubulin; Ubiquitous; K-ALPHA-1; Tubulin Alpha; TBA1B_HUMAN; Tubulin, Alpha 1a; TUBA3; Tubulin Alpha-3 Chain; Tubulin B-Alpha-1; LIS3; Tubulin, Alpha, Brain-Specific; Tubulin Alpha-1A Chain; Alpha-Tubulin 3, Brain-Specific; Hum-A-Tub1; Hum-A-Tub2; B-ALPHA-1; TBA1A_HUMAN;|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 免疫学 细胞骨架|
|交叉反应||Human, Mouse, Rat, Chicken, Pig, Cow,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||50kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human TUBA1A + TUBA1B|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to the mouse and rat Tuba1 genes. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q. Mutations in this gene cause lissencephaly type 3 (LIS3) - a neurological condition characterized by microcephaly, mental retardation, and early-onset epilepsy and caused by defective neuronal migration. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.
Ubiquitously expressed with highest levels in Brain, Bone, Adipocyte, and Breast
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable).
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity).
Belongs to the tubulin family.
Entrez Gene: 7846 Human
Entrez Gene: 22142 Mouse
Entrez Gene: 64158 Rat
Omim: 602529 Human
SwissProt: Q71U36 Human
SwissProt: P68369 Mouse
SwissProt: P68370 Rat
Unigene: 654422 Human
Unigene: 405359 Mouse
Unigene: 234326 Rat
Entrez Gene: 10376 Human
Entrez Gene: 22143 Mouse
Entrez Gene: 500929 Rat
Omim: 602530 Human
SwissProt: P68363 Human
SwissProt: P05213 Mouse
SwissProt: Q6P9V9 Rat
Unigene: 524390 Human
Unigene: 392113 Mouse
Unigene: 99661 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
真核细胞骨架的微管具有基本和多样的功能，由α和β微管的异二聚体组成。编码这些微管成分的基因属于微管蛋白超家族，由六个不同的家族组成。α、β和γ-微管蛋白家族的基因在所有真核生物中都有发现。α和β微管蛋白是微管的主要成分，而γ微管蛋白在微管组装成核中起关键作用。多个α和β微管蛋白基因在物种间高度保守。该基因编码α-微管蛋白，与小鼠和大鼠TUBA1基因高度相似。Northern印迹研究表明，该基因主要表达于形态分化的神经细胞。该基因是染色体12q上的三个α-微管蛋白基因簇中的一个。该基因的突变导致利森脑3型（LIS3）——一种以小脑、智力低下和早期癫痫为特征的神经学疾病，由神经元迁移缺陷引起。选择性剪接导致多个转录变体编码不同的亚型。[ RefSeq，JUL 2012 ]