Rabbit Anti-Anillin/FITC Conjugated antibody
|别 名||Actin binding protein scraps homolog Drosophila; Actin binding protein anillin; Anillin actin binding protein; ANLN; DKFZp779A055; Scra; Scraps; Scraps homolog.|
|规格价格||100ul/2980元 购买 大包装/询价|
|说 明 书||100ul|
|研究领域||细胞生物 细胞周期蛋白 结合蛋白 细胞骨架|
|交叉反应||Human, Mouse, Rat, Dog, Horse,|
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||124kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human Anillin|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis. Before the cell exits into G1, Anillin associates with E-cadherin and is ubiquitinated by the anaphase-promoting complex/cyclosome (APC/C). APC/C recognizes the D-box domain at the N-terminal region of Anillin. Anillin is commonly overexpressed in tumors and may serve as a potential biomarker.Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.
Interacts with F-actin. Interacts with CD2AP. May interact with RHOA. Interacts with FZR1/CDH1 during mitotic exit.
Nucleus. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Note=Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody.
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors.
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1.
Contains 1 PH domain.
Entrez Gene: 54443 Human
Entrez Gene: 68743 Mouse
Entrez Gene: 363031 Rat
SwissProt: Q9NQW6 Human
SwissProt: Q8K298 Mouse
Unigene: 62180 Human
Unigene: 282751 Mouse
Unigene: 453238 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications
Anillin，也被称为碎片同源物，是一种在进化上保守的肌动蛋白结合蛋白，在果蝇黑腹症中首次被鉴定为胞质分裂所必需的。Anillin是一种普遍表达的蛋白，在中枢神经系统中表达水平最高。它主要存在于细胞核中，在胞质分裂过程中定位于解理沟，在Rac GTPase的帮助下形成环。在胞质分裂过程中，Anillin与CD2AP相互作用，起到浓缩Rho A和维持活性肌球蛋白定位的作用。在Anillin敲除细胞中，卵裂沟不能完全侵入。Anillin表达水平随细胞周期而波动，在有丝分裂中达到高峰。在细胞进入G1之前，Anillin与E-cadherin结合，并通过后期促进复合物/环体（APC/C）泛素。APC/C识别Anillin的N端区域的D-盒结构域。Anillin在肿瘤中普遍过表达，可以作为潜在的生物标志物。这对于卵裂沟的结构完整性和卵裂沟进入的完成至关重要。