Anti-IME1抗体上海钰博生物科技有限公司

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产品名称: Anti-IME1抗体
英文名称: IME1
抗体货号: YB--16619R
产品规格: 20ul/50ul/100ul/200ul
级    别: 分析纯, 见说明书
产品产地: 中国/美国
品牌商标: Ybscience
价    格: 1380元
抗原: KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1:301-360/360
抗原来源: Rabbit
抗体来源: Rabbit
适用物种: Rat, Saccharomyces cerevisiae
见说明书conjugate:见说明书
Isotype:见说明书
应用范围: 科研使用
更新时间: 2018/12/6 15:12:00
详细资料:  实验方法技术资料
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使用范围:仅限科研使用,不能应用于临床
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上海钰博生物科技有限公司
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产品详细描述

 Anti-IME1抗体

产品编号 YB-16619R
英文名称 IME1
中文名称 减数分裂诱导蛋白IME1抗体
别    名 Meiosis inducing protein; IME1_YEAST; YJR094C.  
规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
说 明 书 100ul  200ul
研究领域 细胞生物  转录调节因子  表观遗传学  泛素  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Rat, Saccharomyces cerevisiae
产品应用 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 42kDa
细胞定位 细胞核 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1:301-360/360 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression.

Function:
Transcription factor required for sporulation and for early sporulation-specific genes expression. Positive regulator of SME1/IME2 expression. Directly activates expression of SLZ1 during meiosis.

Subunit:
Interacts with UME6.

Subcellular Location:
Nuclear

SWISS:
P21190

Gene ID:
853556

Database links:

Entrez Gene: 853556 Saccharomyces cerevisiae

SwissProt: P21190 Saccharomyces cerevisiae



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
 
产品图片
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; 
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
Incubation: Anti-IME1 Polyclonal Antibody, Unconjugated(bs-16619R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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