Anti-IME1抗体
产品名称: Anti-IME1抗体
英文名称: IME1
产品编号: YB--16619R
产品价格: null
产品产地: 中国/美国
品牌商标: Ybscience
更新时间: 2023-08-17T10:29:50
使用范围: 科研使用
上海钰博生物科技有限公司
- 联系人 : 陈环环
- 地址 : 上海市沪闵路6088号龙之梦大厦8楼806室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 183****2235
- 传真 : 021-60514606
- 邮箱 : shybio@126.com
Anti-IME1抗体
产品编号 | YB-16619R |
英文名称 | IME1 |
中文名称 | 减数分裂诱导蛋白IME1抗体 |
别 名 | Meiosis inducing protein; IME1_YEAST; YJR094C. |
规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 100ul 200ul |
研究领域 | 细胞生物 转录调节因子 表观遗传学 泛素 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Rat, Saccharomyces cerevisiae |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 42kDa |
细胞定位 | 细胞核 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from Saccharomyces cerevisiae IME1:301-360/360 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: Meiosis-inducing protein (IME1) is a master regulator of meiosis that is active only during meiotic events, activates transcription of early meiotic genes through interaction with Ume6p, degraded by the 26S proteasome following phosphorylation by Ime2p. IME1 is required for sporulation and for early sporulation-specific genes expression. It is a positive regulator of SME1/IME2 expression. Function: Transcription factor required for sporulation and for early sporulation-specific genes expression. Positive regulator of SME1/IME2 expression. Directly activates expression of SLZ1 during meiosis. Subunit: Interacts with UME6. Subcellular Location: Nuclear SWISS: P21190 Gene ID: 853556 Database links: Entrez Gene: 853556 Saccharomyces cerevisiae SwissProt: P21190 Saccharomyces cerevisiae Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Tissue/cell: Rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IME1 Polyclonal Antibody, Unconjugated(bs-16619R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |