|别 名||CAG repeat protein 32; CAGH32; DKFZP434I225; Domino homolog; E1A binding protein p400; EP 400; EP400; FLJ42018; FLJ45115; hDomino; KIAA1498; KIAA1818; p400 kDa SWI2/SNF2 related protein; P400 SWI2/SNF2-related protein; TNRC12; Trinucleotide repeat containing 12; Trinucleotide repeat containing gene 12 protein; EP400_HUMAN.|
|规格价格||100ul/1580元 购买 200ul/2480元 购买 大包装/询价|
|说 明 书||100ul 200ul|
|研究领域||肿瘤 细胞生物 免疫学 转录调节因子 表观遗传学|
|交叉反应||Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||344kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human p400:1051-1250/3159|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
NuAR is a multi-protein histone acetyltransferase complex that functions to acetylate the nucleosomal Histones H4 and H2A, thereby activating transcription of select target genes. p400, also known as EP400 (E1A binding protein p400) or mDomino, localizes to the nucleus and is a component of the NuAR complex. Expressed in brain, liver, thymus, lung, spleen, colon and kidney, p400 regulates the transcriptional activity of proteins such as MZF-1 and contributes to the ATPase and helicase activities of NuA4. p400 is a SWI2/ SNF2-related protein that can interact with the adenovirus oncoprotein E1A, thus activating the proapoptotic activity of E1A. The ability of p400 to regulate transcriptional and apoptotic activity suggests that the NuAR complex may be a crucial component of cell proliferation, transformation and, possibly, carcinogenesis. Five isoforms of p400 exist due to alternative splicing events.
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity.
Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. May also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. The NuA4 complex interacts with MYC and the adenovirus E1A protein. EP400 interacts with TRRAP, RUVBL1 and RUVBL2. Interacts with ZNF42. Interacts with PHF5A (By similarity).
Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 Myb-like domain.
Entrez Gene: 57634 Human
Entrez Gene: 75560 Mouse
Omim: 606265 Human
SwissProt: Q96L91 Human
SwissProt: Q8C0P6 Mouse
SwissProt: Q8CHI8 Mouse
Unigene: 306094 Human
Unigene: 595201 Human
Unigene: 270487 Mouse
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications