级 别： 分析纯, 见说明书
价 格： 1580元
抗原： KLH conjugated synthetic peptide derived from human SEMCAP3/LNX3:951-1066/1066
适用物种： Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,
更新时间： 2019/1/14 11:41:00
|别 名||Ligand of Numb protein X 3; likely ortholog of mouse semaF cytoplasmic domain associated protein 3; LNX3; PDZ domain containing ring finger 3; Protein SEMACAP3; SEMACAP3; Semaphorin cytoplasmic domain associated protein; PZRN3_HUMAN.|
|规格价格||100ul/1580元 购买 200ul/2480元 购买 大包装/询价|
|说 明 书||100ul 200ul|
|研究领域||细胞生物 发育生物学 细胞周期蛋白 细胞分化|
|交叉反应||Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,|
|产品应用||ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
|分 子 量||120kDa|
|性 状||Lyophilized or Liquid|
|免 疫 原||KLH conjugated synthetic peptide derived from human SEMCAP3/LNX3:951-1066/1066|
|纯化方法||affinity purified by Protein A|
|储 存 液||0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.|
|保存条件||Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.|
PDZRN3 contains a RING-finger motif in its N-terminal region, two PDZ domains in its central region and a consensus-binding motif for PDZ domains at its C-terminus. It was identified in silico as a homolog of the protein known as LNX1 or SEMCAP1, which possesses ubiquitin ligase activity and binds the membrane protein Semaphorin 4C. However, PDZRN3 itself has not previously been characterized. We have now evaluated the properties and functions of PDZRN3. The PDZRN3 gene was shown to be expressed in various human tissues including the heart, skeletal muscle and liver and its expression in mouse skeletal muscle was developmentally regulated. Both the differentiation of C2C12 mouse skeletal myoblasts into myotubes and injury-induced muscle regeneration in vivo were found to be accompanied by up-regulation of PDZRN3. The differentiation-associated increase in the expression of PDZRN3 in C2C12 cells follows that of myogenin and precedes that of myosin heavy chain. Depletion of PDZRN3 by RNA interference inhibited the formation of myotubes as well as the associated up-regulation of myosin heavy chain in C2C12 cells. Our data suggest that PDZRN3 plays an essential role in the differentiation of myoblasts into myotubes by acting either downstream or independently of myogenin.
E3 ubiquitin-protein ligase. Plays an important role in regulating the surface level of MUSK on myotubes. Mediates the ubiquitination of MUSK, promoting its endocytosis and lysosomal degradation. Might contribute to terminal myogenic differentiation (By similarity).
Interacts with NLGN1 and EFNB2 (By similarity). Interacts with UBE2D2 and with MUSK via the first PDZ domain (By similarity).
SEMCAP3 is widely expressed at high levels, and is thought to be involved in protein/zinc ion binding.
Auto-ubiquitinated (By similarity).
Contains 2 PDZ (DHR) domains.
Contains 1 RING-type zinc finger.
Contains 1 TRAF-type zinc finger.
Entrez Gene: 23024 Human
Entrez Gene: 55983 Mouse
Entrez Gene: 312607 Rat
Omim: 609729 Human
SwissProt: Q9UPQ7 Human
SwissProt: Q69ZS0 Mouse
SwissProt: P68907 Rat
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.