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Product Name: SARS-CoV-2(Omicron)
S1 +S2 trimer Protein（ECD,His Tag）
Cat No. : nCoV-S1
+S2-Omicron
Description: The spike (S)
glycoprotein of coronaviruses contains protrusions that will only bind to
certain receptors on the host cell. Known receptors bind S1 are ACE2,
angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN,
aminopeptidase N; CEACAM, carcinoembryonic
antigen-related cell adhesion molecule 1;
Sia, sialic acid; 0-ac Sia, 0-acetylated sialic acid. The spike is essential
for both host specificity and viral infectivity. The term 'peplomer' is
typically used to refer to a grouping of heterologous proteins on the virus
surface that function together. The spike (S) glycoprotein of coronaviruses is
known to be essential in the binding of the virus to the host cell at the
advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus,
2019-nCoV) can infect the human respiratory epithelial cells through
interaction with the human ACE2 receptor. The spike protein is a large type I
transmembrane protein containing two subunits,
S1 and S2. S1 mainly contains a receptor
binding domain (RBD), which is responsible for recognizing the cell surface receptor.
S2 contains basic elements needed for the membrane fusion. The S protein plays key
parts in the induction of neutralizing-antibody and T-cell responses,
as well as protective immunity. The main functions
for the Spike protein are summarized as: Mediate receptor binding and membrane
fusion;Defines the range of the hosts and specificity of the virus; Main component
to bind with the neutralizing antibody; Key target for vaccine design; Can be
transmitted between different hosts through gene recombination or mutation of
the receptor binding domain (RBD),leading to a higher mortality rate.
Protein Construction: A DNA sequence
encoding the SARS-CoV-2 Spike S1+S2 (YP_ 009724390.1,with mutations A67V,
HV69-70 deletion, T95I, G142D, VYY143-145 deletion,
N211 deletion, L2121, ins214EPE, G339D,
S371L, S373P, S375F, K417N,N440K, G446S, S477N, T478K, E484A, Q493R, G496S,
Q498R, N501Y,Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y,
F817P,N856K, A892P, A899P, A942P, Q954H, N969K, L981F, K986P, V987P and
furin cleavage site mutants) expressed with
the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations
were identified in the SARS-CoV-2 variant (known as variant B.1.1 .529) which
emerged in theSouth Africa.
Sourse:SARS-CoV-2
Expression Host:HEK293 Cells
Purity:>90% as determined by SDS-PAGE
Molecular Mass: The recombinant SARS-CoV-2 Spike S1+S2 consists
of 1228 amino acids and predicts a molecular mass of 136.67 kDa.
Formulation:Supplied as solution form in PBS, pH7.5 or
lyophilized from PBS, pH7.5
Shipping:In general, proteins are shipped out with blue ice
unless customers require otherwise.
Stability &Storage:Use a manual defrost freezer and avoid
repeated freeze thaw cycles.
Store at 2 to 8 °C for one week .
Store at -20 to -80 °C for twelve months from the date of receipt.
SDS-PAGE image:
Note:For research use only .Not for use in clinical
diagnostic procedures.
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