Size  100ul
Cat code  220026
Species Cross-reactivity  H,R
Key Application  WB
Host  Mouse
Clone Number  5B8-D7-C5
Antibody type  Monoclonal antibody
Purified method  Affinity purified
Isotype  IgG1
Molecular Weight  9kDa
Sensitivity  The antibody detects endogenous level of total NEDD8 protein.
Immunogen  Purified recombinant fragment of human NEDD8 expressed in E. Coli.
UniProt Number  Q15843
GeneBank ID  NM_006156.2
Subcellular location  Nucleus. Note: Mainly nuclear.
Formulation  Purified antibody in PBS with 0.05% sodium azide and 0.5% protein stabilizer
Altername  FLJ43224 ; MGC104393 ; MGC125896 ; MGC125897 ; NED8 ; NEDD 8 ; NEDD-8 ; Nedd8 ; NEDD8_HUMAN ; Neddylin ; Neural precursor cell expressed developmentally down regulated 8 ; Neural precursor cell expressed developmentally down regulated gene 8 ; Neural precursor cell expressed developmentally down-regulated protein 8 ; Rub1 ; Ubiquitin like protein Nedd 8 ; Ubiquitin like protein Nedd8 ; Ubiquitin-like protein Nedd8 ;
Gene_symbol  NEDD-8
Summary  
UniPort summary  Function  Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Application Images :  
Western blot detection of NEDD8 antibody in C6 and Hela cell lysates using NEDD8 antibody(dilution 1:500).Predicted band size:90KDa.Observed band size:90KDa.