Size  100ul
Cat code  220033
Species Cross-reactivity  H
Key Application  WB
Host  Mouse
Clone Number  2H9-F11
Antibody type  Monoclonal antibody
Purified method  
Isotype  IgG1
Molecular Weight  52kDa
Sensitivity  The antibody detects endogenous level of total IRAK4 protein.
Immunogen  Purified recombinant fragment of human IRAK4 expressed in E. Coli.
UniProt Number  Q9NWZ3
GeneBank ID  
Subcellular location  Cytoplasm
Formulation  Ascitic fluid containing 0.03% sodium azide.
Altername  IL-1 receptor-associated kinase 4; Interleukin 1 receptor associated kinase 4 mutant form 1; Interleukin-1 receptor-associated kinase 4; Interleukin1 receptor associated kinase 4; IPD1; IRAK 4; IRAK-4; IRAK4; IRAK4 mutated form 1; IRAK4_HUMAN; LOC 51135; NY REN 64; NY REN 64 antigen; NY-REN-64; REN64; Renal carcinoma antigen NY-REN-64;
Gene_symbol  IPD1; REN64; IRAK-4; NY-REN-64
Summary  This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
UniPort summary  Function  Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
Application Images :  
Western blot detection of IRAK4 antibody in MCF7 cell lysates using IRAK4 antibody (dilution 1:500).Predicted band size:52 KDa.Observed band size:55KDa.