ATP5A1抗体
Applications
| In-house applications |
ELISA, IHC, WB, IF Application not tested? Please see our Guarantee. |
| Published applications |
IF, WB |
Publications |
10 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse, rat |
|
Images
Prev12Next
 WB results of 14676-1-AP (anti-ATP5A1 antibody). Immunofluorescent analysis of HepG2 cells, using ATP5A1 antibody 14676-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Immunohistochemical of paraffin-embedded human liver using 14676-1-AP(ATP5A1 antibody) at dilution of 1:100 (under 10x lens) Immunohistochemical of paraffin-embedded human liver using 14676-1-AP(ATP5A1 antibody) at dilution of 1:100 (under 40x lens) Immunohistochemistry of paraffin-embedded human kidney slide using 14676-1-AP( ATP5A1 Antibody) at dilution of 1:50 Immunohistochemistry of paraffin-embedded human kidney slide using 14676-1-AP( ATP5A1 Antibody) at dilution of 1:50
|
| Positive WB detected in |
Multi-cells/tissue, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF7 cells, mouse heart tissue, mouse liver tissue, PC-3 cells, rat heart tissue, Y79 cells |
| Positive IHC detected in |
Human liver tissue, human kidney tissue |
| Positive IF detected in |
HepG2 cells, Hela cells |
| Recommended dilution |
WB : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
ATP5A1抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
ATP5A1 fusion protein ag6385 |
Full name |
ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle |
Calculated molecular
weight |
60 kDa |
Observed molecular
weight |
50-55kd |
GenBank accession
number |
BC064562ATP5A1抗体 |
Gene ID (NCBI) |
498 |
| Gene symbol |
ATP5A1 |
Synonyms |
ATP5A, ATP5A1, ATP5AL2, ATPM, hATP1, MOM2, OMR, ORM |
Background
|
The ATP5A1 gene encodes the α subunit of mitochondrial ATP synthase which produces ATP from ADP in the presence of a proton gradient across the membrane. The mitochondrial ATP synthase, also known as Complex V or F1F0 ATP synthase, is a multi-subunit enzyme complex consisting of two functional domains, the F1-containing the catalytic core and the Fo- containing the membrane proton channel. F0 domain has 10 subunits: a,b, c, d, e, f, g, OSCP, A6L, and F6. F1 is composed of subunits α, β, γ, δ, ε, and a loosely attached inhibitor protein IF1. Recently defect in ATP5A1 has been linked to the fatal neonatal mitochondrial encephalopathy. ATP5A1 is localized in the mitochondria and anti-ATP5A1 can be used as the loading control for mitochondrial or Complex V proteins. This antibody recognizes the endogenous ATP5A1 protein in lysates from various cell lines and tissues.ATP5A1抗体
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