FUS/TLS抗体
Applications
| In-house applications |
ELISA, IF, IP, WB, IHC, FC Application not tested? Please see our Guarantee. |
| Published applications |
IF, IHC, WB |
Publications |
26 citations –view here |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
| Published Species |
Human, mouse |
|
Images
Prev12Next
 WB result of FUS antibody (11570-1-AP, 1:5000) with si-Control and si-FUS transfected HEK 293 cells. HEK-293 cells were subjected to SDS PAGE followed by western blot with 11570-1-AP( FUS/TLS Antibody) at dilution of 1:2000 Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using 11570-1-AP( FUS/TLS Antibody) at dilution of 1:50 (under 40x lens) Immunofluorescent analysis of HepG2 cells, using FUS antibody 11570-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). IP Result of anti-FUS (IP:11570-1-AP, 3ug; Detection:11570-1-AP 1:1000) with K-562 cells lysate 4000ug. 1X10^6 HeLa cells were stained with 0.2ug FUS/TLS antibody (11570-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
|
| Positive WB detected in |
HEK-293 cells, HEK-293, HeLa cells, HepG2 cells, HL-60 cells, human kidney tissue, Jurkat cells, K-562 cells, mouse brain tissue, SMMC-7721 cells |
| Positive IP detected in |
K-562 cells |
| Positive IHC detected in |
Human ovary tumor tissue, human brain tissue, human breast cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
HeLa cells |
| ApplicationNote: |
For IHC detection, 60160-1-Ig (mAB) is better. |
| Recommended dilution |
WB : 1:1000-1:10000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
FUS/TLS抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen |
FUS/TLS fusion protein ag2150 |
Full name |
Fusion (involved in t(12;16) in malignant liposarcoma) |
Calculated molecular
weight |
75 kDa |
Observed molecular
weight |
73 kDa |
GenBank accession
number |
BC026062 |
Gene ID (NCBI) |
2521 |
| Gene symbol |
FUS |
Synonyms |
75 kDa DNA pairing protein, FUS, FUS1, FUS-CHOP, hnRNP P2, Oncogene FUS, Oncogene TLS, POMp75, RNA binding protein FUS, TLS, TLS/CHOP |
Background FUS/TLS抗体
|
FUS (also named TLS and POMp75) belongs to the RRM TET family. FUS may play a role in the maintenance of genomic integrity; it binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. FUS is also an RNA-binding protein, and its links to neurodegenerative disease proffer the intriguing possibility that altered RNA metabolism or RNA processing may underlie or contribute to neuron degeneration[PMID: 22640227]. FUS may be a cause of angiomatoid fibrous histiocytoma (AFH) and is implicated in certain forms of amyotrophic lateral sclerosis (ALS) and frontotemporal dementias (FTDs) such as frontotemporal lobar dementia with ubiquitin inclusions (FTLD-U)[PMID: 22640227]. This antibody is a rabbit polyclonal antibody raised against an internal region of human FUS. FUS/TLS抗体
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