Podoplanin抗体-抗体-抗体-生物在线
Podoplanin抗体

Podoplanin抗体

商家询价

产品名称: Podoplanin抗体

英文名称: PodoplaninAntibody

产品编号: XY11629-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司
  • 联系人 : 徐经理
  • 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
  • 邮编 : 200612
  • 所在区域 : 上海
  • 电话 : 152****8802
  • 传真 : 021-37680378
  • 邮箱 : shxysw02@163.com

 PODOPLANIN抗体Applications

In-house applications ELISA, IHC, WB, FC     Application not tested? Please see our Guarantee.
Published applications IHC Publications 2 citations –view here
Species specificity Human; other species not tested.
Published Species Human
Images
Prev12NextView more validation data
  • 11629-1-AP;HEK-293 cellsHEK-293 cells were subjected to SDS PAGE followed by western blot with 11629-1-AP( PDPN,D2-40,M2A Antibody) at dilution of 1:1000
  • 11629-1-AP;human colon cancer tissueImmunohistochemical of paraffin-embedded human colon cancer using 11629-1-AP(PDPN,D2-40,M2A antibody) at dilution of 1:50 (under 10x lens)
  • 11629-1-AP;human colon cancer tissueImmunohistochemical of paraffin-embedded human colon cancer using 11629-1-AP(PDPN,D2-40,M2A antibody) at dilution of 1:50 (under 40x lens)
  • 11629-1-AP;Transfected HEK-293 cellsTransfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 11629-1-AP( PDPN,D2-40,M2A Antibody) at dilution of 1:1000
  • 11629-1-AP;HUVEC cells1X10^6 HUVEC cells were stained with 0.2ug PDPN,D2-40,M2A antibody (11629-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
Positive WB detected in HEK-293 cells, human placenta tissue, Transfected HEK-293 cells
Positive IHC detected in Human colon cancer tissue, human lung tissue, human skeletal muscle tissue
Positive FC detected in HUVEC cells
Recommended dilution WB : 1:500-1:5000
IHC : 1:20-1:200
 PODOPLANIN抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information PODOPLANIN抗体
Immunogen Podoplanin fusion protein ag2201 Full name Podoplanin
Calculated molecular
weight
17 kDa, 25 kDa Observed molecular 
weight
43 kDa
GenBank accession 
number
BC022812 Gene ID (NCBI) 10630
Gene symbol PDPN Synonyms AGGRUS, D2 40, D2 40,M2A, Glycoprotein 36, GP36, Gp38, GP40, HT1A 1, M2A, OTS8, PA2.26, PA2.26 antigen, PDPN, podoplanin, PSEC0025, T1 alpha, T1A, T1A 2
Background

M2A(D2-40) antigen is a previously identified marker for CIS and seminoma. M2A (D2-40) antigen has been identical to PDPN.(PMID: 16736189). PDPN, also named as GP36, Gp38, GP40, HT1A-1, OTS8, PA2.26, T1A and T1A-2, has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin. PODOPLANIN抗体

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