Capicua,Capicua蛋白抗体

产品编号HZ-11721R
英文名称Capicua
中文名称Capicua蛋白抗体
别 名Capicua homolog (Drosophila); Cic; CIC; KIAA0306; Protein capicua homolog; CIC_HUMAN.
说 明 书0.1ml 0.2ml
研究领域神经生物学 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,
Capicua,Capicua蛋白抗体产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量164kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Capicua (1475-1560aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Capicua,Capicua蛋白抗体PubMedPubMed
产品介绍background:
Capicua is the mammalian ortholog of the drosophilia Cic gene and is part of the HMG-box protein superfamily. Expressed primarily in the fetal brain, Capicua functions as a transciptional repressor and is involved in the development of the nervous system through interaction with the ATXN1 protein. When ATXN1 assembles into stable complexes, it directly binds Capicua, thereby mediating both the activity and expression of Capicua. When Capicua is active, it is able to interact with other developmental proteins to restrict the growth of granule cells and regulate normal neuronal development. Disruptions in the the association of Capicua with proteins such as ATXN1 are thought to cause medulloblastoma, the most common form of perdiatric brain tumor arising from irregular growth of granule cells.

Function:
Transcriptional repressor which may play a role in development of the central nervous system (CNS).

Subunit:
Interacts with ATXN1 and ATXN1L

Subcellular Location:
Nucleus.

Tissue Specificity:
Expressed in fetal brain.

Similarity:
Contains 1 HMG box DNA-binding domain.

Database links:
Entrez Gene: 23152 Human
SwissProt: Q96RK0 Human
Unigene: 388236 Human

Capicua,Capicua蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: brain of mouse embryo; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Capicua Polyclonal Antibody, Unconjugated(HZ-11721R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining