NSP5/NSP 5 alpha 3 alpha,核结构蛋白5抗体

产品编号HZ-7816R
英文名称NSP5/NSP 5 alpha 3 alpha
中文名称核结构蛋白5抗体
别 名Cytokinesis and spindle organization B; NSP 5 alpha 3 alpha; Cytospin B; FLJ36955; HCMOGT 1; NSP; NSP5; Nuclear structure protein 5; SPECC1; Spectrin domain with coiled coils 1; Sperm antigen HCMOGT 1; Sperm antigen with calponin homology and coiled coil domains 1; Structure protein NSP5a3a; Structure protein NSP5a3b; Structure protein NSP5b3a; Structure protein NSP5b3b; CYTSB_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 细胞周期蛋白 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Rat, Dog, Rabbit, Sheep,
NSP5/NSP 5 alpha 3 alpha,核结构蛋白5抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量119kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human NSP5
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
NSP5/NSP 5 alpha 3 alpha,核结构蛋白5抗体PubMedPubMed
产品介绍background:
NSP type 5 alpha 3 alpha is a novel structure protein belonging to the cytospin-A family, that may play a role in nuclear function during cell division. It is also highly expressed in certain tumor cell lines, suggesting that it may serve as a good tumor marker. It was was cloned as part of a chromosomal translocation involving PDGFRB in a case of juvenile myelomonocytic leukemia.

Subcellular Location:
Nucleus.

Tissue Specificity:
Highly expressed in testis. Barely detectablein other tissues. Also highly expressed in some cancer cell lines.

DISEASE:
Note=A chromosomal aberration involving CYTSB may be acause of juvenile myelomonocytic leukemia. Translocationt(5;17)(q33;p11.2) with PDGFRB.

Similarity:
Belongs to the cytospin-A family.
Contains 1 CH (calponin-homology) domain.

NSP5/NSP 5 alpha 3 alpha,核结构蛋白5抗体Database links:
Entrez Gene: 92521 Human
Omim: 608793 Human
SwissProt: Q5M775 Human
Unigene: 431045 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: Rat brain tisue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-NSP5 Polyclonal Antibody, Unconjugated(HZ-7816R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining