MNAT1,细胞周期G1的相互作用蛋白抗体

产品编号HZ-7896R
英文名称MNAT1
中文名称细胞周期G1的相互作用蛋白抗体
别 名MNAT 1; CAP35; CDK activating kinase assembly factor MAT1; CDK7/cyclin H assembly factor; Cyclin G1 interacting protein; MAT1; Menage a trois; MNAT 1; p35; p36; RING finger protein 66; RING finger protein MAT1; RNF66; MAT1_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导 细胞周期蛋白 激酶和磷酸酶 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
MNAT1,细胞周期G1的相互作用蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量36kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human MNAT1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
MNAT1,细胞周期G1的相互作用蛋白抗体PubMedPubMed
产品介绍background:
MNAT1 (or 'menage a trois-1') is a subunit of the CAK complex which is a multisubunit protein that includes CDK7, cyclin H, as well as MNAT1. CAK (CDK-activating kinase) phosphorylates and thus activates CDKS (Cyclin-dependent kinases) which play an essential role in cell cycle control of eukaryotic cells.

Function:
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II.

Subunit:
Associates primarily with CDK7 and cyclin H to form the CAK complex. CAK can further associate with the core-TFIIH to form the TFIIH basal transcription factor.

MNAT1,细胞周期G1的相互作用蛋白抗体Subcellular Location:
Nucleus.

Tissue Specificity:
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes.

Similarity:
Contains 1 RING-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.

Database links:
Entrez Gene: 4331 Human
Entrez Gene: 17420 Mouse
Entrez Gene: 266713 Rat
Omim: 602659 Human
SwissProt: P51948 Human
SwissProt: P51949 Mouse
Unigene: 509523 Human
Unigene: 246750 Mouse
Unigene: 15168 Rat

MNAT1,细胞周期G1的相互作用蛋白抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片	Protein: mouse muscle lysate; Primary: rabbit Anti-MNAT1 (HZ-7896R) at 1:300;  Secondary: HRP conjugated Goat-Anti-rabbit IgG(HZ-0295G-HRP) at 1: 5000;  Predicted band size:36 kD Observed band size:39 kD