PLEKHM2,血小板白细胞C激酶底物同源结构域M2抗体

产品编号HZ-8063R
英文名称PLEKHM2
中文名称血小板白细胞C激酶底物同源结构域M2抗体
别 名PH domain containing family M member 2; Pleckstrin homology domain containing family M (with RUN domain) member 2; Pleckstrin homology domain containing family M member 2; PLEKHM 2; RP11 169K16.1; Salmonella induced filaments A and kinesin interacting protein; SifA (Salmonella induced filaments A) and kinesin interacting protein; SifA and kinesin interacting protein; SKIP; KIAA0842; Novel RUN and PH domain containing protein; PKHM2_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 细菌及病毒
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Cow, Horse,
PLEKHM2,血小板白细胞C激酶底物同源结构域M2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量113kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human PLEKHM2
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PLEKHM2,血小板白细胞C激酶底物同源结构域M2抗体PubMedPubMed
产品介绍background:
PLEKHM2, also known as SKIP, is a member of the M family of Pleckstrin homology domain-containing proteins. While little is known of PLEKHM2, a recent study examining differential gene expression in human hematopoietic stem cells has shown it to be specifically expressed in stem cells, suggesting that PLEKHM2 may play a role in erythroid commitment and development. Other studies have shown that PLEKHM2 is required for interaction with the Salmonella virulence factor SifA for Salmonella pathogenesis, suggesting that PLEKHM2 has cellular roles other than in the developing embryo.

Function:
May play a role in the regulation of conventional kinesin activity. Required for maintenance of the Golgi apparatus organization. May play a role in membrane tubulation.

Subunit:
Interacts with KIF5B. Interacts with the S.typhimurium sifA protein; required for S.typhimurium infection.

Subcellular Location:
Cytoplasm.

PLEKHM2,血小板白细胞C激酶底物同源结构域M2抗体Similarity:
Contains 1 PH domain.
Contains 1 RUN domain.

Database links:
UniProtKB/Swiss-Prot: Q8IWE5.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-PLEKHM2/SKIP Polyclonal Antibody, Unconjugated(HZ-8063R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining