Spindly,卷曲螺旋结构域蛋白99抗体

产品编号HZ-2321R
英文名称Spindly
中文名称卷曲螺旋结构域蛋白99抗体
别 名Rrhabdomyosarcoma antigen protein MU RMS 40.4A; Arsenite related gene 1 protein; Arsenite-related gene 1 protein; CCDC 99; Ccdc99; Coiled coil domain containing 99; Coiled-coil domain-containing protein 99; hSpindly; Protein Spindly; rhabdomyosarcoma antigen MU RMS 40.4A; Rhabdomyosarcoma antigen MU-RMS-40.4A; SPDLY_HUMAN; Spindly.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Pig, Cow,
Spindly,卷曲螺旋结构域蛋白99抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量67kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Protein Spindly
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Spindly,卷曲螺旋结构域蛋白99抗体PubMedPubMed
产品介绍background:
The five integral families of plant hormones consists of auxins, cytokinins, giberellins (GAs), abscisic acid (ABA), and ethylene. Giberellins, which consist of over fifty family members, mediate shoot growth. In Arabidopsis, SPINDLY (SPY) negatively regulates GA signal transduction. ERA1 (enhanced response to absisic acid), which is identical to WIGGUM, controls floral and shoot apical meristem size and floral organ number in response to ABA. Ethylene is perceived by a family of five receptors, one of which is ETR1, whereas CTR1 is a negative regulator of the ethylene signal transduction pathway . Ethylene is also produced endogenously in Arabidopsis via a biosynthetic pathway, which is catalyzed by ACC synthase and ACC oxidase.

Function:
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.

Spindly,卷曲螺旋结构域蛋白99抗体Subcellular Location:
Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.

Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:
Belongs to the Spindly family.

Gene ID:
54908

Database links:
Entrez Gene: 54908 Human
SwissProt: Q96EA4 Human
Unigene: 368710 Human

Spindly,卷曲螺旋结构域蛋白99抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品介绍

background: The five integral families of plant hormones consists of auxins, cytokinins, giberellins (GAs), abscisic acid (ABA), and ethylene. Giberellins, which consist of over fifty family members, mediate shoot growth. In Arabidopsis, SPINDLY (SPY) negatively regulates GA signal transduction. ERA1 (enhanced response to absisic acid), which is identical to WIGGUM, controls floral and shoot apical meristem size and floral organ number in response to ABA. Ethylene is perceived by a family of five receptors, one of which is ETR1, whereas CTR1 is a negative regulator of the ethylene signal transduction pathway . Ethylene is also produced endogenously in Arabidopsis via a biosynthetic pathway, which is catalyzed by ACC synthase and ACC oxidase. Function: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Subcellular Location: Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the Spindly family. Gene ID: 54908 Database links: Entrez Gene: 54908 Human SwissProt: Q96EA4 Human Unigene: 368710 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: human rectal carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Spindly/CCDC99 Polyclonal Antibody, Unconjugated(HZ-2321R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(HZ-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Spindly Polyclonal Antibody, Unconjugated(HZ-2321R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Spindly Polyclonal Antibody, Unconjugated(HZ-2321R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining