Anti-HRAS (G13A) Mouse Monoclonal Antibody-抗体-抗体-生物在线
Anti-HRAS (G13A) Mouse Monoclonal Antibody

Anti-HRAS (G13A) Mouse Monoclonal Antibody

商家询价

产品名称： Anti-HRAS (G13A) Mouse Monoclonal Antibody

英文名称： Anti-HRAS (G13A) Mouse Monoclonal Antibody

产品编号： 26191

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC

武汉纽斯特生物技术有限公司
  • 联系人 :
  • 地址 : 武汉市东湖新技术开发区光谷生物城B3-3栋2-3楼
  • 邮编 : 430074
  • 所在区域 : 湖北
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  • 邮箱 : neweast3@126.com
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Ras (G13A) [26191]
[100ul]		  

Catalog Number:  26191
Gene Symbol:  H-Ras; K-Ras; N-Ras
Description:   Anti-HRAS (G13A) Mouse Monoclonal Antibody
Background:   Belongs to the small GTPase superfamily. Ras family binds GDP/GTP and possess intrinsic GTPase activity, alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).Located in cell membrane, lipid-anchor, cytoplasmic side and Golgi apparatus membrane.
Immunogen:   A synthetic peptide from the internal region of HRas which includes the mutation of G13A, human origin.
Applications:   ELISA, WB, IHC
Recommended Dilutions:   
ELISA: 1:1000-1:5000
WB: 1:500-1:1000
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Mouse
Format:   Liquid
Clonality:   Monoclonal
Isotype:   
Purity:   Purified from ascites
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Recognizes G13A mutant, but not wild-type H-RAS of vertebrates.
Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of recombinant HRas (G13A) and wildtype proteins. Purified His-tagged HRas (G13A) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti-HRas (G13A) monoclonal antibody (Cat. #26191).
Immunofluorescence:   
Immunofluorescence of cells expressing Ras proteins with anti-HRas (G13A) antibody. HEK293T cells were transfected with pCDNA3-GFP-HRas (WT) plasmid (left column) or pCDNA3-GFP-HRas (G13A) plasmid (right column), then fixed and stained with anti-HRas (G13A) monoclonal antibody (Cat. #26191).

 

Publications:
1.  DNA vaccine elicits an efficient antitumor response by targeting the mutant Kras in a transgenic mouse lung cancer model
    Gene Therapy 21, 888-896 (October 2014)
2.  Are all RAS mutations the same? Coexisting KRAS and NRAS mutations in a