Anti-SUMO-2/3 Rabbit polyclonal Antibody-抗体-抗体-生物在线
Anti-SUMO-2/3 Rabbit polyclonal Antibody

Anti-SUMO-2/3 Rabbit polyclonal Antibody

商家询价

产品名称： Anti-SUMO-2/3 Rabbit polyclonal Antibody

英文名称： Anti-SUMO-2/3 Rabbit polyclonal Antibody

产品编号： 21237

产品价格： null

产品产地： 美国

品牌商标： NewEastbio

更新时间： null

使用范围： ELISA, WB, IHC, IP

武汉纽斯特生物技术有限公司
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  • 所在区域 : 湖北
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  • 邮箱 : neweast3@126.com
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SUMO-2/3 [21237]
[100 µL]		  

Catalog Number:  21237
Gene Symbol:  SUMO2/3
Description:   Anti-SUMO-2/3 Rabbit polyclonal Antibody
Background:   Small Ubiquitin-like Modifier or SUMO proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle. There are 3 confirmed SUMO isoforms in humans: SUMO-1, SUMO-2 and SUMO-3. SUMO-2 and -3 show a high degree of similarity to each other and are distinct from SUMO-1.
Immunogen:   A synthetic peptide corresponding to residues near the C-terminal of human SUMO-2/3.
Applications:   ELISA, WB, IHC, IP
Recommended Dilutions:   
ELISA: 1:1000-1:5000 
WB: 1:100-1:1000 
IHC: 1:50-1:100
Concentration:   1 mg/ml
Host:   Rabbit
Format:   Liquid
Clonality:   Polyclonal
Isotype:   
Purity:   Purified from serum
Storage buffer:
Preservative:  no
Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity:  Detects SUMO-2/3 but not SUMO-1 from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
 
Western blot:  
Western blot analysis of over-expressed SUMO-2 and SUMO-3 proteins. HEK293 cells were transfected with SUMO-2 (lane 2) and SUMO-3 (lane 3) cDNAs and control empty vector (lane 1). Cell lysates were blotted with anti-SUMO-2/3 rabbit polyclonal antibody (Cat. # 21237).
 
Publications:
1.  The SUMOylation of zinc-fingers and homeoboxes 1 (ZHX1) by Ubc9 regulates its stability and transcriptional repression activity
    Journal of Cellular Biochemistry Volume 114, Issue 10, pages 2323–2333, October 2013
2.  C-terminal Src Kinase (Csk)-mediated Phosphorylation of Eukaryotic Elongation Factor 2 (eEF2) Promotes Proteolytic Cleavage and Nuclear Translocation of eEF2
    J Biol Chem. 2014 May 2;289(18):12666-78