Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2
产品名称： Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2
英文名称： Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin
产品编号： IC-103-01
产品价格： null
产品产地： 美国
品牌商标： XY-Bioscience
更新时间： 2023-08-17T09:55:27
使用范围： > 98 % by SDS-PAGE and HPLC analyses.
上海信裕生物科技有限公司
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Recombinant Human Tumor Necrosis Factor-alpha/TNFSF2
| Synonyms | Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin |
| Accession | P01375 |
| GeneID | 7124 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids. |
| Quantity | 5µg/100µg/1mg |
| AA Sequence | MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 10 mM NaCl, pH 7.0. |
| Endotoxin | Less than 0.01 EU/μg of rHuTNF-α/TNFSF2 GMP as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage | This GMP product can be for research use or further manufacturing use. |
| Quality statement | The manufacture and testing of this product is in compliance with ICH Q7a guidelines. |
| Reference | 1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9. 2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483. 3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41. 4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202. |
| Background | Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types |