Recombinant Human Keratinocyte Growth Factor-2/FGF-10
产品名称： Recombinant Human Keratinocyte Growth Factor-2/FGF-10
英文名称： rHuKGF-2/FGF-10
产品编号： IC-104-10
产品价格： null
产品产地： 美国
品牌商标： XY-Bioscience
更新时间： 2023-08-17T09:55:27
使用范围： > 97 % by SDS-PAGE and HPLC analyses.
上海信裕生物科技有限公司
- 联系人 : 徐经理
- 地址 : 上海市闵行莘庄工业区春东路508号A1-2F
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 152****8802
- 传真 : 021-37680378
- 邮箱 : shxysw02@163.com
Recombinant Human Keratinocyte Growth Factor-2/FGF-10
| Species | 9 |
| Accession | O15520 |
| GeneID | 2255 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids. |
| Quantity | 5µg/25µg/1000µg |
| AA Sequence | LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/µg of rHuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Usage | This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE. |
| SDS-PAGE |  |
| Reference | 1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4. 2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404. 3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63. 4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5. 5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7. |
| Background | Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant human FGF-10 contains a 208 amino acids and it shares 92 % and 95 % amino acid sequence identity with murine and rat FGF-10. Defects in FGF-10 are the cause of autosomal dominant aplasia of lacrimal and salivary glands and lacrimo-auriculo-dento-digital syndrome |