小鼠抗O6甲基鸟嘌呤DNA甲基转移酶单克隆抗体
产品名称： 小鼠抗O6甲基鸟嘌呤DNA甲基转移酶单克隆抗体
英文名称： MGMT
产品编号： hzm-51234M
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:20-100 IHC-F=1:20-100 ICC=1:100-500 IF=1:20-100
上海沪震实业有限公司
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749
- 传真 : 021-60345367
- 邮箱 : www.shzbio.net
Mouse Anti-MGMT antibody
| 产品编号 | hzm-51234M |
| 英文名称 | MGMT |
| 中文名称 | 小鼠抗O6甲基鸟嘌呤DNA甲基转移酶单克隆抗体 |
| 别 名 | 6 O methylguanine DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT: O-6 methylguanine DNA methyltransferase; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6 methylguanine DNA alkyltransferase; O-6-methylguanine-DNA methyltransferase; O6 methylguanine DNA methyltransferase; MGMT_HUMAN. |
| 研究领域 | 肿瘤 转录调节因子 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4C12 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 ELISA=1:100-1000 IHC-P=1:20-100 IHC-F=1:20-100 ICC=1:100-500 IF=1:20-100 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 22kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human MGMT: |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: MGMT (O6 methylguanine DNA methyltransferase) is an enzyme that transfers methyl groups from O(6) methylguanine, and other methylated moities of DNA, to a cysteine residue in itself, thus repairing DNA damage caused by alkylation in a single step reaction. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT prevents mutagenesis and malignant transformation, and also provides resistance to chemotherapy with alkylating agents among cancer patients. Function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Subcellular Location: Nucleus. Similarity: Belongs to the MGMT family. SWISS: P16455 Gene ID: 4255 Database links: Entrez Gene: 4255 Human Omim: 156569 Human SwissProt: P16455 Human Unigene: 501522 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |