磷酸化整合素连接激酶1抗体
产品名称： 磷酸化整合素连接激酶1抗体
英文名称： phospho-ILK-1(Ser259)
产品编号： hz-5445R
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
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Rabbit Anti-phospho-ILK-1(Ser259) antibody
| 产品编号 | hz-5445R |
| 英文名称 | phospho-ILK-1(Ser259) |
| 中文名称 | 磷酸化整合素连接激酶1抗体 |
| 别 名 | ILK-1 (phospho S259); ILK-1 (phospho Ser259); p-ILK-1 (Ser259); 59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; ILK-2; ILK_HUMAN; Integrin linked Kinase; Integrin-linked protein kinase; DKFZp686F1765; EC 2.7.11.1; ILK 1; ILK 2; ILK; ILK1; ILK2; Integrin linked Kinase 2; Integrin linked protein kinase; p59; p59ILK. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 免疫学 神经生物学 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 50kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human ILK-1 around the phosphorylation site of Ser259:CQ(p-S)PP |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active. Function: Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. Subunit: Interacts with cytoplasmic domain of beta 1 subunit of integrin. Could also interacts with beta 2, beta 3 and/or beta 5 subunit of integrin. Interacts (via ANK repeats) with LIMS1 and LIMS2. Interacts with parvins and probably TGFB1I1. Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1. Subcellular Location: Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Tissue Specificity: Highly expressed in heart followed by skeletal muscle, pancreas and kidney. Weakly expressed in placenta, lung and liver. Post-translational modifications: Autophosphorylated on serine residues. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 5 ANK repeats. Contains 1 protein kinase domain. SWISS: Q13418 Gene ID: 3611 Database links: Entrez Gene: 3611 Human Entrez Gene: 16202 Mouse Entrez Gene: 170922 Rat Omim: 602366 Human SwissProt: Q13418 Human SwissProt: O55222 Mouse SwissProt: Q99J82 Rat Unigene: 5158 Human Unigene: 706355 Human Unigene: 274846 Mouse Unigene: 95042 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ILK 是一种新发现的Ser/Thr蛋白激酶。ILK能够通过与整合素β1亚单位的结合介导细胞与胞外基质的连接，以依赖于PI3K的方式激活，并通过磷酸化下游底物PKB/AKT,GSK3等胞外信号的一项下游传递，对细胞的生长，分化，迁移等进行调控。由于ILK在胞内外信号传导中起着重要的作用。并且抑制ILK的活性能够导致细胞周期的停滞和细胞程序性死亡的启动，使其成为肿瘤治疗和肿瘤药物的理想靶位点 |