磷酸化3磷酸肌醇依赖性蛋白激酶1抗体-抗体-抗体-生物在线
磷酸化3磷酸肌醇依赖性蛋白激酶1抗体

磷酸化3磷酸肌醇依赖性蛋白激酶1抗体

商家询价

产品名称: 磷酸化3磷酸肌醇依赖性蛋白激酶1抗体

英文名称: Phospho-PDPK1(Ser410)

产品编号: hz-5545R

产品价格: null

产品产地: 中国/上海

品牌商标: HZbscience

更新时间: 2023-08-17T10:24:20

使用范围: ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500

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 Rabbit Anti-Phospho-PDPK1(Ser410) antibody

 

产品编号 hz-5545R
英文名称 Phospho-PDPK1(Ser410)
中文名称 磷酸化3磷酸肌醇依赖性蛋白激酶1抗体
别    名 PDPK1 (phospho S410)p-PDPK1 (phospho S410); PDPK1(phospho S410); 3 phosphoinositide dependent protein kinase 1; hPDK 1; hPDK1; MGC20087 MGC35290; PDK 1; PDPK 1; PDPK1; PkB kinase; PkB kinase like gene 1; PkB like 1; PkB like; PRO0461; PDPK1_HUMAN.  
   
产品类型 磷酸化抗体 
研究领域 神经生物学  信号转导  细胞凋亡  转录调节因子  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
交叉反应 Human, Mouse, Rat, 
产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 61kDa
细胞定位 细胞核 细胞浆 细胞膜 
性    状 Lyophilized or Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human PDPK1 around the phosphorylation site of Ser410:SG(p-S)NI 
亚    型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed PubMed
产品介绍 background:
PDK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase and the stimulation of glycogen synthesis. Because of the role that PDK plays in insulin-induced glycogen synthesis and PKC activation it is a potentially important target for metabolic drug research. There are three named isoforms.

Function:
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

Subunit:
Interacts with NPRL2.

Subcellular Location:
Cytoplasm. Membrane; Peripheral membrane protein. Note=Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.

Tissue Specificity:
Appears to be expressed ubiquitously.

Post-translational modifications:
Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
Contains 1 PH domain.
Contains 1 protein kinase domain.

Gene ID:
5170

Database links:

Entrez Gene: 5170 Human

Entrez Gene: 18607 Mouse

Entrez Gene: 81745 Rat

Omim: 605213 Human

SwissProt: O15530 Human

SwissProt: Q9Z2A0 Mouse

SwissProt: O55173 Rat

Unigene: 459691 Human

Unigene: 10504 Mouse

Unigene: 10905 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

PDK1是蛋白激酶B(PKB)的上游激酶,通过与3,4,5三磷酸磷脂酰肌醇作用激活相邻的PKB分子.PDK1能够激活包含PKB在内的一系列的AGC激酶家族成员.从而调节细胞代谢,生长,扩散,生存,抗凋亡等诸多生理过程