组蛋白赖氨酸N-甲基转移酶3C抗体
产品名称: 组蛋白赖氨酸N-甲基转移酶3C抗体
英文名称: KMT3C
产品编号: hz-16796R
产品价格: null
产品产地: 中国/上海
品牌商标: HZbscience
更新时间: 2023-08-17T10:24:20
使用范围: WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
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Rabbit Anti-KMT3C antibody
产品编号 | hz-16796R |
英文名称 | KMT3C |
中文名称 | 组蛋白赖氨酸N-甲基转移酶3C抗体 |
别 名 | Histone methyltransferase SMYD2; HSKM B; HSKM-B; HSKMB; KMT3C; Lysine N-methyltransferase 3C; MGC119305; N lysine methyltransferase SMYD2; N-lysine methyltransferase SMYD2; SET and MYND domain containing 2; SET and MYND domain containing protein 2; SET and MYND domain-containing protein 2; Smyd2; SMYD2_HUMAN; Zinc finger MYND domain containing 14; ZMYND14. |
产品类型 | 甲基化抗体 |
研究领域 | 转录调节因子 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, Sheep, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 50kDa |
细胞定位 | 细胞核 细胞浆 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human KMT3C:351-433/433 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: SET domain-containing proteins, such as SMYD2, catalyze lysine methylation (Brown et al., 2006 [PubMed 16805913]).[supplied by OMIM, Nov 2008] Function: Protein-lysine N-methyltransferase that methylates both histones and non-histone proteins. Specifically methylates histone H3 'Lys-4' (H3K4me) and dimethylates histone H3 'Lys-36' (H3K36me2). Has also methyltransferase activity toward non-histone proteins such as p53/TP53 and RB1. Monomethylates 'Lys-370' of p53/TP53, leading to decreased DNA-binding activity and subsequent transcriptional regulation activity of p53/TP53. Monomethylates 'Lys-860' of RB1/RB. Subunit: Interacts with RNA polymerase II and HELZ. Interacts with SIN3A and HDAC1 (By similarity). Interacts (via MYND-type zinc finger) with EPB41L3. Interacts (via SET domain) with p53/TP53. Interacts with RB1 and HSP90AA1. Subcellular Location: Cytoplasm; cytosol. Nucleus. Similarity: Contains 1 MYND-type zinc finger. Contains 1 SET domain. SWISS: Q9NRG4 Gene ID: 56950 Database links: Entrez Gene: 56950 Human Entrez Gene: 615229 Cow Entrez Gene: 226830 Mouse Entrez Gene: 100294706 Pig Entrez Gene: 289372 Rat Omim: 610663 Human SwissProt: Q0P585 Cow SwissProt: Q9NRG4 Human SwissProt: Q8R5A0 Mouse SwissProt: C3RZA1 Pig SwissProt: Q7M6Z3 Rat Unigene: 66170 Human Unigene: 156895 Mouse Unigene: 7052 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品图片 |
Sample: heart (mouse) Lysate at 40 ug
Primary: Anti-KMT3C(bs-16796R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50 kD Observed band size: 50 kD Tissue/cell: Human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min; Incubation: Anti-KMT3C Polyclonal Antibody, Unconjugated(bs-16796R) 1:500, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |